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PMID: 14582203 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of bacterial beta-galactosidase in animal cells.

Molecular and cellular biology ·Vol. 2 ·No. 12 ·1982-12-00 ·Pages 1628-32

An G, Hidaka K, Siminovitch L

Abstract

A recombinant plasmid containing the gene for bacterial beta-galactosidase, situated close to the simian virus 40 early promoter, has been constructed. Transfection of CHO, L, and COS-1 cells with this plasmid led to the expression and appearance of the enzyme. Using this system, we have developed a series of promoter cloning vehicles capable of accepting promoter signals for animal genes.

MeSH Terms
Animals Bacterial Proteins/biosynthesis,genetics Cell Line Escherichia coli/genetics Gene Expression Regulation Genes, Bacterial Plasmids/genetics,metabolism Promoter Regions, Genetic Recombinant Proteins/biosynthesis beta-Galactosidase/biosynthesis,genetics
Chemicals
Bacterial Proteins Recombinant Proteins beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
An G
Department of Medical Genetics, University of Toronto, Hospital for Sick Children, Toronto, Ontario, Canada.
Hidaka K
Siminovitch L
References (20)
20 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.2.12.1628-1632.1982
Published
1982-12-00
Pages
1628-32
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369970
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.2.12.1628-1632.1982 PMC Full Text View journal details
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