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PMID: 189079 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Synthesis of Epstein-Barr virus DNA in vitro: effects of phosphonoacetic acid, N-ethylmaleimide, and ATP.

Journal of virology ·Vol. 21 ·No. 1 ·1977-01-00 ·Pages 435-8

Seebeck T, Shaw JE, Pagano JS

Abstract

Nuclei from superinfected Raji cells synthesized Epstein-Barr Virus (EBV) DNA in vitro in the absence of cell DNA synthesis. The synthesis of EBV DNA in vitro was inhibited by phosphonoacetic acid and N-ethylmaleimide, and maximum synthesis was achieved in the absence of an ATP-regenerating system. Nuclei from mock-infected cells required an ATP-regenerating system for maximum DNA synthesis.

MeSH Terms
Acetates/pharmacology Adenosine Triphosphate/metabolism Cell Line Cell Nucleus/metabolism Cell-Free System DNA Replication/drug effects DNA, Viral/biosynthesis Ethylmaleimide/pharmacology Herpesvirus 4, Human/metabolism Organophosphorus Compounds/pharmacology
Chemicals
Acetates DNA, Viral Organophosphorus Compounds Adenosine Triphosphate Ethylmaleimide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Seebeck T
Shaw J E
Pagano J S
References (10)
10 references, click to expand
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    Proc Natl Acad Sci U S A. 1976 May;73(5):1745-8 PMID: 179098
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    Virology. 1976 May;71(1):352-4 PMID: 179207
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    J Virol. 1972 Apr;9(4):714-6 PMID: 4336566
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.21.1.435-438.1977
Published
1977-01-00
Pages
435-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353834
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.21.1.435-438.1977 PMC Full Text View journal details
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