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PMID: 166387 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Viral and cellular DNA synthesis in nuclei from human lymphocytes transformed by Epstein-Barr virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 6 ·1975-06-00 ·Pages 2413-7

Benz WC, Strominger JL

Abstract

A DNA-synthesizing system in vitro, using nuclei prepared by treatment of human lymphocytes with the detergent Brij 58, was developed. Nuclei from cultured lymphocytes synthesized DNA for as long as 5 hr, and required ATP, deoxynucleoside triphosphates, magnesium, and a calcium chelator. In nuclei from a partially synchronized line of cultured lymphocytes carrying several hundred copies of the Epstein-Barr viral genome, synthesis in vitro was predominately viral in early S phase and cellular in late S phase. These and other data suggested that the DNA synthesis observed in vitro was predominately replicative.

MeSH Terms
Adenosine Triphosphate/pharmacology Bromodeoxyuridine/metabolism Cell Fractionation Cell Line Cell Nucleus/drug effects,metabolism Centrifugation, Density Gradient DNA/biosynthesis DNA, Viral/biosynthesis Detergents/pharmacology Egtazic Acid/pharmacology Herpesvirus 4, Human Humans Kinetics Lectins/pharmacology Lymphocyte Activation Lymphocytes/drug effects,metabolism,ultrastructure Magnesium/pharmacology
Chemicals
DNA, Viral Detergents Lectins Egtazic Acid Adenosine Triphosphate DNA Bromodeoxyuridine Magnesium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Benz W C
Strominger J L
References (30)
30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.6.2413
Published
1975-06-00
Pages
2413-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432769
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.6.2413 PMC Full Text View journal details
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