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PMID: 960726 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Preferential inhibition of herpes-group viruses by phosphonoacetic acid: effect on virus DNA synthesis and virus-induced DNA polymerase activity.

The Yale journal of biology and medicine ·Vol. 49 ·No. 1 ·1976-03-00 ·Pages 93-9

Huang ES, Huang CH, Huong SM, Selgrade M

Abstract

In tissue culture phosphonoacetic acid (PAA) specifically inhibited DNA synthesis of human cytomegalovirus (CMV), murine CMV, simian CMV, Epstein-Barr virus, and Herpesvirus saimiri. Fifty to one hundred micrograms per milliliter PAA completely inhibited viral DNA synthesis with no significant damage to host cell DNA synthesis. In vitro DNA polymerization assays showed that 10 μg/ml of PAA specifically inhibited partially purified human CMV-induced DNA polymerase, while little inhibition of host-cell DNA polymerase activity was found. The specific inhibition of herpes-group virus DNA synthesis with little toxicity to host cells suggests that PAA has great potential as an antiherpesvirus therapeutic agent.

MeSH Terms
Acetates/pharmacology Culture Techniques DNA Nucleotidyltransferases/metabolism DNA, Viral/biosynthesis Herpesviridae/metabolism Organophosphorus Compounds/pharmacology Virus Replication
Chemicals
Acetates DNA, Viral Organophosphorus Compounds DNA Nucleotidyltransferases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Huang E S
Huang C H
Huong S M
Selgrade M
References (11)
11 references, click to expand
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Article Info
Journal
The Yale journal of biology and medicine
Abbr.
Yale J Biol Med
ISSN
0044-0086
Published
1976-03-00
Pages
93-9
Language
English
Region
United States
NLM ID
0417414
PMCID
PMC2595326
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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