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PMID: 179098 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Differential effect of phosphonoacetic acid on the expression of Epstein-Barr viral antigens and virus production.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 5 ·1976-05-00 ·Pages 1745-8

Nyormoi O, Thorley-Lawson DA, Elkington J, Strominger JL

Abstract

The effects of phosphonoacetic acid on cell growth, expression of Epstein-Barr virus antigens, and virus production in human and marmoset lymphoblastoid cell lines have been studied. The drug had no significant effect at concentrations up to 100 mug/ml on cell growth or total cell DNA synthesis. Higher doses induced not only a drastic decrease in DNA synthesis and cell grwoth, but also a dramatic cell enlargement. Immunofluorescence studies showed that greater than or equal to 30 mug/ml of phosphonoacetic acid inhibited viral capsid antigen synthesis without affecting the expression of the nuclear antigen or the spontaneous and 5-iodo-2'-deoxyuridine-induced early antigens. Production of transforming Epstein-Barr virus was also blocked.

MeSH Terms
Acetates/pharmacology Antigens, Viral Antiviral Agents/pharmacology Cell Division/drug effects Cell Line Cell Transformation, Neoplastic DNA/biosynthesis DNA, Viral/biosynthesis Herpesvirus 4, Human/growth & development,immunology Organophosphorus Compounds/pharmacology Viral Proteins/biosynthesis Virus Replication/drug effects
Chemicals
Acetates Antigens, Viral Antiviral Agents DNA, Viral Organophosphorus Compounds Viral Proteins DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nyormoi O
Thorley-Lawson D A
Elkington J
Strominger J L
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.5.1745
Published
1976-05-00
Pages
1745-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430377
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.5.1745 PMC Full Text View journal details
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