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PMID: 1846147 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A novel rho promoter::Tn10 mutation suppresses and ftsQ1(Ts) missense mutation in an essential Escherichia coli cell division gene by a mechanism not involving polarity suppression.

Journal of bacteriology ·Vol. 173 ·No. 2 ·1991-01-00 ·Pages 655-63

Storts DR, Markovitz A

Abstract

An extragenic suppressor of the Escherichia coli cell division gene ftsQ1(Ts) was isolated. The suppressor is a Tn10 insertion into the -35 promoter consensus sequence of the rho gene, designated rho promoter::Tn10. The ftsQ1(Ts) mutation was also suppressed by the rho-4 mutant allele. The rho promoter::Tn10 strain does not exhibit rho mutant polarity suppressor phenotypes. In addition, overexpression of the ftsQ1(Ts) mutation does not reverse temperature sensitivity. Furthermore, DNA sequence analysis of the ftsQ1(Ts) allele revealed that the salt-remediable, temperature-sensitive phenotype arose from a single missense mutation. The most striking phenotype of the rho promoter::Tn10 mutant strain is an increase in the level of negative supercoiling. On the basis of these observations, we conclude that the ftsQ1(Ts) mutation may be suppressed by a change in supercoiling.

Related Genes
Is1OR ddl ftsQ ftsQ1(Ts)
MeSH Terms
Base Sequence Cell Division DNA Transposable Elements Escherichia coli/genetics,growth & development Genes, Bacterial Genotype Molecular Sequence Data Mutation Oligonucleotide Probes Phenotype Plasmids Promoter Regions, Genetic Restriction Mapping Rho Factor/genetics Suppression, Genetic Transduction, Genetic
Chemicals
DNA Transposable Elements Oligonucleotide Probes Rho Factor
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Storts D R
Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637.
Markovitz A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.2.655-663.1991
Published
1991-01-00
Pages
655-63
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207057
Subset
IM
Grants
NIAID NIH HHS · AI06966 · United States
External Links
PubMed source DOI: 10.1128/jb.173.2.655-663.1991 PMC Full Text View journal details
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