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PMID: 6350821 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

On the process of cellular division in Escherichia coli: nucleotide sequence of the gene for penicillin-binding protein 3.

Molecular & general genetics : MGG ·Vol. 191 ·No. 1 ·1983-00-00 ·Pages 1-9

Nakamura M, Maruyama IN, Soma M, Kato J, Suzuki H, Horota Y

Abstract

We determined the nucleotide sequence of a DNA fragment containing the ftsI gene coding for the penicillin-binding protein 3 (PBP-3), an indispensable enzyme for cell division of Escherichia coli. The entire ftsI gene was within the 2.8 kilobase PvuII fragment derived from the chromosomal segment on pLC26-6 (Nishimura et al. 1977). The coding region for PBP-3 was identified by comparison with the N-terminal amino acid sequence of in vitro synthesized PBP-3. The structural gene for ftsI consisted of 1,764 base-pairs coding for a 588 amino acid residue-polypeptide with a molecular weight of 63,850. PBP-3 synthesized in vitro showed a lower mobility in SDS-gel electrophoresis than that of the authentic PBP-3, suggesting that the primary translation product of the ftsI gene may be processed to yield mature PBP-3.

MeSH Terms
Bacterial Proteins Base Sequence Carrier Proteins/genetics Cell Division DNA, Bacterial/analysis Escherichia coli/genetics Escherichia coli Proteins Genes Genes, Bacterial Hexosyltransferases Molecular Weight Muramoylpentapeptide Carboxypeptidase Penicillin-Binding Proteins Peptidoglycan Glycosyltransferase Peptidyl Transferases
Chemicals
Bacterial Proteins Carrier Proteins DNA, Bacterial Escherichia coli Proteins FtsI protein, E coli Penicillin-Binding Proteins Peptidyl Transferases Hexosyltransferases Peptidoglycan Glycosyltransferase Muramoylpentapeptide Carboxypeptidase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Nakamura M
Maruyama I N
Soma M
Kato J
Suzuki H
Horota Y
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42 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330881
Published
1983-00-00
Pages
1-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
K00137
External Links
PubMed source DOI: 10.1007/BF00330881
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