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PMID: 331323 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Simultaneous deletion of D-alanine carboxypeptidase IB-C and penicillin-binding component IV in a mutant of Escherichia coli K12.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 7 ·1977-07-00 ·Pages 2980-4

Iwaya M, Strominger JL

Abstract

Mutants of Escherichia coli with much decreased activity of D-alanine carboxypeptidase (peptidyl-D alanine hydrolase, EC 3.4.12.11) were found among E. coli K12 extensively mutagenized with nitrosoguanidine treatment by assaying individual colonies for the enzyme activity. One such mutant with only 10-12% residual activity was characterized extensively. The soluble carboxypeptidase activity (corresponding to D-alanine carboxypeptidase IC of Tamura T., Imae, Y. & Strominger, J.L. [(1976) J. Biol. Chem. 251, 414-423] was deleted. This enzyme activity in the particulate fraction was markedly reduced but transpeptidase activity was normal. However, penicillin-binding component IV was deleted from the particulate fraction. Both the physiology and penicillin sensitivity of the organism were relatively normal, except that mutant cells were markedly more stable to penicillin-induced lysis, suggesting the possibility that carboxypeptidase IC really functions as an endopeptidase. The possible relationship of the deleted carboxypeptidase activity and the deleted penicillin binding component are discussed.

MeSH Terms

Ampicillin/pharmacology Carboxypeptidases/deficiency Carrier Proteins Chromosome Aberrations Chromosome Deletion Deoxycholic Acid/pharmacology Escherichia coli/drug effects,enzymology,metabolism Muramoylpentapeptide Carboxypeptidase/deficiency Mutation Penicillin G/metabolism Subcellular Fractions/enzymology

Chemicals

Carrier Proteins Deoxycholic Acid Ampicillin Carboxypeptidases Muramoylpentapeptide Carboxypeptidase Penicillin G

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Iwaya M

Strominger J L

References (20)

20 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.74.7.2980

Published

1977-07-00

Pages

2980-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC431372

Subset

External Links

PubMed source DOI: 10.1073/pnas.74.7.2980 PMC Full Text View journal details

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Simultaneous deletion of D-alanine carboxypeptidase IB-C and penicillin-binding component IV in a mutant of Escherichia coli K12.

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