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PMID: 1754397 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interaction of DNA-binding proteins with a milk protein gene promoter in vitro: identification of a mammary gland-specific factor.

Nucleic acids research ·Vol. 19 ·No. 23 ·1991-12-11 ·Pages 6603-10

Watson CJ, Gordon KE, Robertson M, Clark AJ

Abstract

The minimal 5' regulatory region of the sheep beta-lactoglobulin gene (BLG), as defined in transgenic mice, was used to identify nuclear factors which may be involved in milk protein gene expression in the lactating mammary gland. This 406bp promoter region was dissected into short, overlapping, double-stranded oligonucleotides to facilitate identification of the bound proteins. A variety of sites, for both known and previously undescribed DNA-binding proteins, are occupied in vitro. Some of these factors were investigated in detail. Two forms of nuclear factor I (NFI), which have different recognition site affinities, are present in nuclear extracts from lactating mammary gland and bind to at least 5 sites in this BLG control element. In addition, a factor (milk protein binding factor, MPBF) which is specific to extracts from both mouse and sheep lactating mammary gland binds to 3 BLG promoter sites and may be a milk protein gene transcription factor.

Related Genes
BLG
MeSH Terms
Animals Base Sequence Binding Sites DNA/metabolism DNA-Binding Proteins/metabolism Lactoglobulins/genetics,metabolism Mammary Glands, Animal/metabolism Mice Molecular Sequence Data Organ Specificity Promoter Regions, Genetic Sheep
Chemicals
DNA-Binding Proteins Lactoglobulins DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Watson C J
AFRC Institute of Animal Physiology and Genetics Research, Edinburgh Research Station, Roslin, Midlothian, UK.
Gordon K E
Robertson M
Clark A J
References (36)
36 references, click to expand
  1. Nuclear proteins from lactating mammary glands bind to the promoter of a milk protein gene.
    Nucleic Acids Res. 1987 Mar 11;15(5):2103-21 PMID: 3031588
  2. Multiple hormone interactions in the developmental biology of the mammary gland.
    Physiol Rev. 1980 Oct;60(4):1049-106 PMID: 7001510
  3. The TGGCA protein binds to the MMTV-LTR, the adenovirus origin of replication, and the BK virus enhancer.
    Nucleic Acids Res. 1985 Mar 25;13(6):2045-61 PMID: 2987840
  4. Organization and sequence of the human alpha-lactalbumin gene.
    Biochem J. 1987 Mar 15;242(3):735-42 PMID: 2954544
  5. In vivo stimulation of a chimeric promoter by binding sites for nuclear factor I.
    Mol Cell Biol. 1991 Jun;11(6):2946-51 PMID: 1903836
  6. Synergistic transcriptional activation by CTF/NF-I and the estrogen receptor involves stabilized interactions with a limiting target factor.
    Mol Cell Biol. 1991 Jun;11(6):2937-45 PMID: 2038313
  7. Developmental regulation of the sheep beta-lactoglobulin gene in the mammary gland of transgenic mice.
    Dev Genet. 1991;12(4):299-307 PMID: 1718646
  8. Two regions of the mouse mammary tumor virus long terminal repeat regulate the activity of its promoter in mammary cell lines.
    Mol Cell Biol. 1991 May;11(5):2529-37 PMID: 1708094
  9. Isolation of complementary DNAs encoding a cerebellum-enriched nuclear factor I family that activates transcription from the mouse myelin basic protein promoter.
    J Biol Chem. 1990 Nov 5;265(31):19065-70 PMID: 1699939
  10. NF-I proteins from brain interact with the proenkephalin cAMP inducible enhancer.
    Nucleic Acids Res. 1991 May 25;19(10):2721-8 PMID: 1828294
  11. Identification of a potent adipocyte-specific enhancer: involvement of an NF-1-like factor.
    Genes Dev. 1991 Mar;5(3):428-37 PMID: 2001842
  12. Activation domains of stably bound GAL4 derivatives alleviate repression of promoters by nucleosomes.
    Cell. 1991 Feb 8;64(3):533-44 PMID: 1991320
  13. Transcription factor ATF interacts with the TATA factor to facilitate establishment of a preinitiation complex.
    Cell. 1988 Sep 23;54(7):1033-42 PMID: 3416354
  14. The estrogen receptor binds tightly to its responsive element as a ligand-induced homodimer.
    Cell. 1988 Oct 7;55(1):145-56 PMID: 3167974
  15. Prolactin and glucocorticoid hormones synergistically induce expression of transfected rat beta-casein gene promoter constructs in a mammary epithelial cell line.
    Proc Natl Acad Sci U S A. 1989 Jan;86(1):104-8 PMID: 2643093
  16. The estrogen-responsive element as an inducible enhancer: DNA sequence requirements and conversion to a glucocorticoid-responsive element.
    EMBO J. 1987 Dec 1;6(12):3719-27 PMID: 3480798
  17. O-glycosylation of eukaryotic transcription factors: implications for mechanisms of transcriptional regulation.
    Cell. 1988 Oct 7;55(1):125-33 PMID: 3139301
  18. Purification of a NF1-like DNA-binding protein from rat liver and cloning of the corresponding cDNA.
    EMBO J. 1988 Oct;7(10):3115-23 PMID: 3053160
  19. Cooperative binding of steroid hormone receptors contributes to transcriptional synergism at target enhancer elements.
    Cell. 1989 May 5;57(3):443-8 PMID: 2566384
  20. Clusters of nuclear factor I binding sites identify enhancers of several papillomaviruses but alone are not sufficient for enhancer function.
    Nucleic Acids Res. 1989 May 11;17(9):3519-33 PMID: 2542901
  21. Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins.
    Science. 1989 Jul 28;245(4916):371-8 PMID: 2667136
  22. Growth control and differentiation in mammary epithelial cells.
    Environ Health Perspect. 1989 Mar;80:85-99 PMID: 2647487
  23. Gene regulation by steroid hormones.
    Cell. 1989 Feb 10;56(3):335-44 PMID: 2644044
  24. The comparative pharmacokinetics of H1-receptor antagonists.
    Ann Allergy. 1987 Dec;59(6 Pt 2):20-4 PMID: 2892445
  25. Conserved region of the rat alpha-lactalbumin promoter is a target site for protein binding in vitro.
    Biochem J. 1988 Dec 1;256(2):391-6 PMID: 3223918
  26. Multiple genes encode nuclear factor 1-like proteins that bind to the promoter for 3-hydroxy-3-methylglutaryl-coenzyme A reductase.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):8963-7 PMID: 3194401
  27. Complete nucleotide sequence of the genomic ovine beta-lactoglobulin gene.
    Nucleic Acids Res. 1988 Nov 11;16(21):10379-80 PMID: 3194215
  28. A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs.
    Nature. 1988 Jul 21;334(6179):218-24 PMID: 3398920
  29. A quantitative analysis of nuclear factor I/DNA interactions.
    Nucleic Acids Res. 1988 May 25;16(10):4419-35 PMID: 3380685
  30. The interplay of DNA-binding proteins on the promoter of the mouse albumin gene.
    Cell. 1987 Dec 24;51(6):963-73 PMID: 3690666
  31. Alteration of the quality of milk by expression of sheep beta-lactoglobulin in transgenic mice.
    Nature. 1987 Aug 6-12;328(6130):530-2 PMID: 3614356
  32. Nuclear factor I acts as a transcription factor on the MMTV promoter but competes with steroid hormone receptors for DNA binding.
    EMBO J. 1990 Jul;9(7):2233-9 PMID: 2162764
  33. Analysis of multiple forms of nuclear factor I in human and murine cell lines.
    Mol Cell Biol. 1990 Mar;10(3):1041-8 PMID: 2304457
  34. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  35. Specific binding of a cellular DNA replication protein to the origin of replication of adenovirus DNA.
    Proc Natl Acad Sci U S A. 1983 Oct;80(20):6177-81 PMID: 6336326
  36. Contactpoint analysis of the HeLa nuclear factor I recognition site reveals symmetrical binding at one side of the DNA helix.
    EMBO J. 1987 Jan;6(1):161-8 PMID: 3034574
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.23.6603
Published
1991-12-11
Pages
6603-10
Language
English
Region
England
NLM ID
0411011
PMCID
PMC329228
Subset
IM
Databases
GENBANK
S72181, S72298, X12817, X60655, X60963, X60964, X60965, X60966, X60967, X60968, X60969
External Links
PubMed source DOI: 10.1093/nar/19.23.6603 PMC Full Text View journal details
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