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PMID: 3223918 Published · ppublish English Journal Article

Conserved region of the rat alpha-lactalbumin promoter is a target site for protein binding in vitro.

The Biochemical journal ·Vol. 256 ·No. 2 ·1988-12-01 ·Pages 391-6

Lubon H, Hennighausen L

Abstract

Nuclear extracts from mammary glands of lactating rats contain DNA binding protein(s) with high affinity to the region -125 to -85 of the rat alpha-lactalbumin promoter. The protected sequence is part of a mammary conserved sequence described by Hall et al. [(1987) Biochem. J. 242, 735-742]. Comparison of the DNA protein-binding sites in the 5' flanking sequence of the rat alpha-lactalbumin and mouse whey acidic protein genes suggests that the consensus sequence 5'-TGGCARNNWCKKC-3' is the protein(s) recognition site. This binding site is part of a highly conserved region [consensus sequence, TGGCAGSCTCGGCST(G)YTCTCTCT(NTG)TGGCARA] present in the promoter of four whey protein genes.

MeSH Terms
Animals Base Sequence Binding, Competitive DNA-Binding Proteins/metabolism Female Lactalbumin/genetics Mammary Glands, Animal/metabolism Mice Milk Proteins/genetics Nuclear Proteins/metabolism Pregnancy Promoter Regions, Genetic Protein Binding Rats Whey Proteins
Chemicals
DNA-Binding Proteins Milk Proteins Nuclear Proteins Whey Proteins Lactalbumin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lubon H
Laboratory of Biochemistry and Metabolism, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892.
Hennighausen L
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2560391
Published
1988-12-01
Pages
391-6
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1135422
Subset
IM
Databases
GENBANK
UNKNOWN
External Links
PubMed source DOI: 10.1042/bj2560391 PMC Full Text View journal details
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