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PMID: 1738200 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Functional characterization of a U5 ribozyme: intracellular suppression of human immunodeficiency virus type 1 expression.

Journal of virology ·Vol. 66 ·No. 3 ·1992-03-00 ·Pages 1432-41

Dropulić B, Lin NH, Martin MA, Jeang KT

Abstract

We have designed a ribozyme that cleaves human immunodeficiency virus type 1 (HIV-1) RNA in U5 (at nucleotide +115). This ribozyme was tested in vitro and was found to give efficient and specific digestion of RNA containing the HIV-1 U5 sequence. When the U5 ribozyme was placed into the HIV-1 genome, virus replication was suppressed in tissue culture. Introduction of this ribozyme into cells by using an amphotropic retrovirus vector significantly reduced expression of U5-containing RNA in cells chronically infected with HIV-1. Naive T cells were cocultivated with packaging cells that produce defective amphotropic retroviruses containing the U5 ribozyme. These lymphocytes were found to be partially protected from HIV-1 infection.

MeSH Terms
Base Sequence Cells, Cultured Cloning, Molecular DNA, Recombinant Gene Expression Regulation, Viral HIV-1/genetics Humans In Vitro Techniques Molecular Sequence Data Oligodeoxyribonucleotides/chemistry RNA, Catalytic/genetics RNA, Viral/chemistry,genetics Substrate Specificity Virus Replication
Chemicals
DNA, Recombinant Oligodeoxyribonucleotides RNA, Catalytic RNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dropulić B
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Lin N H
Martin M A
Jeang K T
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.3.1432-1441.1992
Published
1992-03-00
Pages
1432-41
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240867
Subset
IM
Grants
NIAID NIH HHS · K08 AI081545 · United States
External Links
PubMed source DOI: 10.1128/JVI.66.3.1432-1441.1992 PMC Full Text View journal details
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