Home LiteratureArticle Details
PMID: 2479828 Published · ppublish English Journal Article

Ribozyme, antisense RNA, and antisense DNA inhibition of U7 small nuclear ribonucleoprotein-mediated histone pre-mRNA processing in vitro.

Molecular and cellular biology ·Vol. 9 ·No. 10 ·1989-10-00 ·Pages 4479-87

Cotten M, Schaffner G, Birnstiel ML

Abstract

A comparative analysis of ribozyme, antisense RNA, and antisense DNA inhibitors of the in vitro small nuclear ribonucleoprotein U7-dependent histone pre-mRNA processing reaction was performed. RNA molecules complementary to the U7 sequence inhibited in vitro processing of histone pre-mRNA at a sixfold excess over U7. Single-stranded DNA complementary to the entire U7 sequence inhibited the reaction at a 60-fold excess over U7, while a short, 18-nucleotide DNA molecule complementary to the 5' end of U7 inhibited the processing reaction at a 600-fold excess. A targeted ribozyme was capable of specifically cleaving the U7 small nuclear ribonucleoprotein in a nuclear extract and inhibited the U7-dependent processing reaction, but in our in vitro system it required a 1,000-fold excess over U7 for complete inhibition of processing.

MeSH Terms
Animals Cell-Free System DNA/pharmacology DNA, Antisense Histones/genetics Mice RNA/pharmacology RNA Precursors/metabolism RNA Processing, Post-Transcriptional/drug effects,physiology RNA, Antisense RNA, Catalytic RNA, Ribosomal/genetics,physiology Ribonucleoproteins/metabolism Ribonucleoproteins, Small Nuclear
Chemicals
DNA, Antisense Histones RNA Precursors RNA, Antisense RNA, Catalytic RNA, Ribosomal Ribonucleoproteins Ribonucleoproteins, Small Nuclear RNA DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cotten M
Institute for Molecular Pathology, Vienna, Austria.
Schaffner G
Birnstiel M L
References (27)
27 references, click to expand
  1. Functional analysis of the sea urchin U7 small nuclear RNA.
    Mol Cell Biol. 1988 Mar;8(3):1076-84 PMID: 2835659
  2. An unwinding activity that covalently modifies its double-stranded RNA substrate.
    Cell. 1988 Dec 23;55(6):1089-98 PMID: 3203381
  3. Self-cleavage of virusoid RNA is performed by the proposed 55-nucleotide active site.
    Cell. 1987 Jul 3;50(1):9-16 PMID: 3594567
  4. The Tetrahymena ribozyme acts like an RNA restriction endonuclease.
    Nature. 1986 Dec 4-10;324(6096):429-33 PMID: 3537808
  5. Compensatory mutations suggest that base-pairing with a small nuclear RNA is required to form the 3' end of H3 messenger RNA.
    Nature. 1986 Oct 30-Nov 5;323(6091):777-81 PMID: 3022153
  6. The cDNA sequences of the sea urchin U7 small nuclear RNA suggest specific contacts between histone mRNA precursor and U7 RNA during RNA processing.
    EMBO J. 1984 Dec 1;3(12):2801-7 PMID: 6084590
  7. Cleavage of specific sites of RNA by designed ribozymes.
    FEBS Lett. 1988 Nov 7;239(2):285-8 PMID: 2460373
  8. Biochemical complementation with RNA in the Xenopus oocyte: a small RNA is required for the generation of 3' histone mRNA termini.
    Cell. 1983 Oct;34(3):823-8 PMID: 6194891
  9. Specific contacts between mammalian U7 snRNA and histone precursor RNA are indispensable for the in vitro 3' RNA processing reaction.
    EMBO J. 1988 Mar;7(3):801-8 PMID: 3396543
  10. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  11. Heat-labile regulatory factor is required for 3' processing of histone precursor mRNAs.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):8937-40 PMID: 2962194
  12. Techniques for using antisense oligodeoxyribonucleotides to study gene expression.
    Anal Biochem. 1988 Aug 1;172(2):289-95 PMID: 3056098
  13. Antisense DNA and RNA: progress and prospects.
    Genes Dev. 1988 May;2(5):502-4 PMID: 2454867
  14. Identification of the human U7 snRNP as one of several factors involved in the 3' end maturation of histone premessenger RNA's.
    Science. 1987 Dec 18;238(4834):1682-7 PMID: 2825355
  15. Oligonucleotide analogues as potential chemotherapeutic agents.
    Pharm Res. 1988 Sep;5(9):539-49 PMID: 3073387
  16. Structural and functional characterization of mouse U7 small nuclear RNA active in 3' processing of histone pre-mRNA.
    Mol Cell Biol. 1988 Apr;8(4):1518-24 PMID: 3380087
  17. Construction of a series of several self-cleaving RNA duplexes using synthetic 21-mers.
    FEBS Lett. 1988 Feb 15;228(2):228-30 PMID: 2449365
  18. Sequence-specific endoribonuclease activity of the Tetrahymena ribozyme: enhanced cleavage of certain oligonucleotide substrates that form mismatched ribozyme-substrate complexes.
    Biochemistry. 1988 Dec 13;27(25):8924-31 PMID: 3069131
  19. snRNP mediators of 3' end processing: functional fossils?
    Trends Biochem Sci. 1988 Nov;13(11):447-51 PMID: 2908086
  20. Satellite tobacco ringspot virus RNA: A subset of the RNA sequence is sufficient for autolytic processing.
    Proc Natl Acad Sci U S A. 1986 Dec;83(23):8859-62 PMID: 16593780
  21. Self-cleaving viroid and newt RNAs may only be active as dimers.
    Nature. 1988 Jul 21;334(6179):265-7 PMID: 2456468
  22. Conserved terminal hairpin sequences of histone mRNA precursors are not involved in duplex formation with the U7 RNA but act as a target site for a distinct processing factor.
    Proc Natl Acad Sci U S A. 1989 Jun;86(12):4345-9 PMID: 2734288
  23. Genetic complementation in the Xenopus oocyte: co-expression of sea urchin histone and U7 RNAs restores 3' processing of H3 pre-mRNA in the oocyte.
    EMBO J. 1986 Jul;5(7):1675-82 PMID: 2943587
  24. Generation of histone mRNA 3' ends by endonucleolytic cleavage of the pre-mRNA in a snRNP-dependent in vitro reaction.
    EMBO J. 1986 Jun;5(6):1319-26 PMID: 3015597
  25. Transcription termination and 3' processing: the end is in site!
    Cell. 1985 Jun;41(2):349-59 PMID: 2580642
  26. A small catalytic oligoribonucleotide.
    Nature. 1987 Aug 13-19;328(6131):596-600 PMID: 2441261
  27. Simple RNA enzymes with new and highly specific endoribonuclease activities.
    Nature. 1988 Aug 18;334(6183):585-91 PMID: 2457170
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.10.4479-4487.1989
Published
1989-10-00
Pages
4479-87
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362532
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.10.4479-4487.1989 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2