Cleavage of specific sites of RNA by designed ribozymes.

FEBS letters ·Vol. 239 ·No. 2 ·1988-11-07 ·Pages 285-8

Koizumi M, Iwai S, Ohtsuka E

Abstract

Two ribozymes were designed for site-specific cleavage of RNA. A UA site in an undecaribonucleotide was cleaved by a ribozyme consisting of two partially paired oligoribonucleotides with chain lengths of 19 and 15. The other ribozyme, which consists of a 19-mer and a 13-mer, recognized a UC sequence at positions 42 and 43 of 5 S rRNA.

MeSH Terms

Base Sequence Molecular Sequence Data Nucleic Acid Conformation RNA/metabolism RNA Splicing RNA, Catalytic RNA, Ribosomal/metabolism RNA, Ribosomal, 5S/metabolism Substrate Specificity

Chemicals

RNA, Catalytic RNA, Ribosomal RNA, Ribosomal, 5S RNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Koizumi M

Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.

Iwai S

Ohtsuka E

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/0014-5793(88)80935-9

Published

1988-11-07

Pages

285-8

Language

English

Region

England

NLM ID

0155157

Subset

External Links

PubMed source DOI: 10.1016/0014-5793(88)80935-9 View journal details

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Cleavage of specific sites of RNA by designed ribozymes.

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