Home LiteratureArticle Details
PMID: 1985211 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inhibition of human immunodeficiency virus type 1 replication in human T cells stably expressing antisense RNA.

Journal of virology ·Vol. 65 ·No. 1 ·1991-01-00 ·Pages 468-72

Sczakiel G, Pawlita M

Abstract

Human T-lymphoid cell lines with constitutive intracellular expression of antisense RNA complementary to a 407-bp sequence of the 5' leader-gag region of human immunodeficiency virus type 1 were established by using a nonretroviral expression vector. In cell lines with antisense RNA expression detectable by Northern (RNA) hybridization, human immunodeficiency virus type 1 replication was inhibited to 88% 10 days postinfection and this inhibition lasted 3 weeks postinfection.

MeSH Terms
Cell Line Cell Transformation, Viral Chromosomes, Human Clone Cells HIV-1/genetics,physiology Humans Kinetics RNA, Antisense/genetics RNA, Viral/genetics T-Lymphocytes Time Factors Transfection Virus Replication
Chemicals
RNA, Antisense RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sczakiel G
Institut für Virusforschung, Deutsches Krebsforschungszentrum, Heidelberg, Federal Republic of Germany.
Pawlita M
References (28)
28 references, click to expand
  1. Inhibition of human immunodeficiency virus type 1-mediated cytopathic effects by poly(L-lysine)-conjugated synthetic antisense oligodeoxyribonucleotides.
    J Gen Virol. 1989 Oct;70 ( Pt 10):2673-82 PMID: 2794975
  2. Inhibition of human immunodeficiency virus (HIV-1) replication by synthetic oligo-RNA derivatives.
    Nucleic Acids Res. 1989 Jan 11;17(1):239-52 PMID: 2911465
  3. Antisense RNA and DNA.
    Sci Am. 1990 Jan;262(1):40-6 PMID: 1688469
  4. Ribozymes as potential anti-HIV-1 therapeutic agents.
    Science. 1990 Mar 9;247(4947):1222-5 PMID: 2107573
  5. Phosphate-methylated DNA aimed at HIV-1 RNA loops and integrated DNA inhibits viral infectivity.
    Science. 1990 Apr 13;248(4952):208-12 PMID: 2326635
  6. Cloning and functional analysis of multiply spliced mRNA species of human immunodeficiency virus type 1.
    J Virol. 1990 Jun;64(6):2519-29 PMID: 2335812
  7. Specific inhibition of human immunodeficiency virus type 1 replication by RNA transcribed in sense and antisense orientation from the 5'-leader/gag region.
    Biochem Biophys Res Commun. 1990 Jun 15;169(2):643-51 PMID: 1694076
  8. Inhibition of Rous sarcoma virus replication and cell transformation by a specific oligodeoxynucleotide.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):280-4 PMID: 75545
  9. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma.
    Int J Cancer. 1977 May 15;19(5):621-6 PMID: 68013
  10. Inhibition of human immunodeficiency virus replication in cell culture by endogenously synthesized antisense RNA.
    J Gen Virol. 1990 Sep;71 ( Pt 9):1965-74 PMID: 2170567
  11. Inhibition of Rous sarcoma viral RNA translation by a specific oligodeoxyribonucleotide.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):285-8 PMID: 75546
  12. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2072-6 PMID: 7017722
  13. Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS.
    Science. 1984 May 4;224(4648):497-500 PMID: 6200935
  14. Complete nucleotide sequence of the AIDS virus, HTLV-III.
    Nature. 1985 Jan 24-30;313(6000):277-84 PMID: 2578615
  15. Nucleic acid structure and expression of the human AIDS/lymphadenopathy retrovirus.
    Nature. 1985 Feb 7-13;313(6002):450-8 PMID: 2982104
  16. A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus.
    Cell. 1985 Jun;41(2):521-30 PMID: 2985280
  17. A novel immune system against bacteriophage infection using complementary RNA (micRNA).
    Nature. 1985 Jun 13-19;315(6020):601-3 PMID: 2409446
  18. Inhibition of replication and expression of human T-cell lymphotropic virus type III in cultured cells by exogenous synthetic oligonucleotides complementary to viral RNA.
    Proc Natl Acad Sci U S A. 1986 Jun;83(12):4143-6 PMID: 3012555
  19. Inhibition of retroviral replication by anti-sense RNA.
    Mol Cell Biol. 1986 Dec;6(12):4758-62 PMID: 2432406
  20. Inhibition of Rous sarcoma virus replication by antisense RNA.
    J Virol. 1987 Mar;61(3):921-4 PMID: 2433470
  21. Efficient replication of HTLV-III and STLV-IIImac in human Jurkat cells.
    Med Microbiol Immunol. 1987;176(5):273-80 PMID: 2443825
  22. Phosphorothioate analogs of oligodeoxynucleotides: inhibitors of replication and cytopathic effects of human immunodeficiency virus.
    Proc Natl Acad Sci U S A. 1987 Nov;84(21):7706-10 PMID: 3499613
  23. Antisense RNA inhibition of endogenous genes.
    Methods Enzymol. 1987;151:519-30 PMID: 2448580
  24. Antisense DNA and RNA: progress and prospects.
    Genes Dev. 1988 May;2(5):502-4 PMID: 2454867
  25. Inhibition of human immunodeficiency virus replication by antisense oligodeoxynucleotides.
    Proc Natl Acad Sci U S A. 1988 Aug;85(15):5507-11 PMID: 3041414
  26. Gene therapy. Intracellular immunization.
    Nature. 1988 Sep 29;335(6189):395-6 PMID: 3166513
  27. Electrotransfection of human lymphoid and myeloid cell lines.
    Nucleic Acids Res. 1988 Dec 23;16(24):11840 PMID: 3211764
  28. Inhibition of human immunodeficiency virus in early infected and chronically infected cells by antisense oligodeoxynucleotides and their phosphorothioate analogues.
    Proc Natl Acad Sci U S A. 1989 Oct;86(20):7790-4 PMID: 2682627
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.1.468-472.1991
Published
1991-01-00
Pages
468-72
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240540
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.65.1.468-472.1991 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2