Efficient replication of HTLV-III and STLV-IIImac in human Jurkat cells.

Medical microbiology and immunology ·Vol. 176 ·No. 5 ·1987-00-00 ·Pages 273-80

Wendler I, Jentsch KD, Schneider J, Hunsmann G

Abstract

High producer cell lines were established by infecting Jurkat cells with either HTLV-IIIB from H9 cells or with STLV-IIImac from HUT-78 cells. The Jurkat cells produced both viruses at least 10 times more efficiently then the original cell lines; the pelleted virus from Jurkat cells was also less contaminated with non-virion proteins. Accordingly, a higher specificity was attained in an ELISA for antibodies to HTLV-III if the antigenic activity was derived from the virus from Jurkat cells, as opposed to that from H9 cells.

MeSH Terms

Acquired Immunodeficiency Syndrome/immunology Animals Antibody Specificity Antigens, Viral/immunology Cell Line HIV/growth & development Humans Leukemia, Lymphoid Macaca mulatta/microbiology RNA-Directed DNA Polymerase/analysis T-Lymphocytes/microbiology Viral Proteins/analysis Virus Replication

Chemicals

Antigens, Viral Viral Proteins RNA-Directed DNA Polymerase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Wendler I

Deutsches Primatenzentrum, Göttingen, Federal Republic of Germany.

Jentsch K D

Schneider J

Hunsmann G

References (21)

21 references, click to expand

Surface expression of murine leukemia virus structural polypeptides on host cells and the virion.
Int J Cancer. 1978 Aug 15;22(2):204-13 PMID: 81184
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Isolation of lymphocytopathic retroviruses from San Francisco patients with AIDS.
Science. 1984 Aug 24;225(4664):840-2 PMID: 6206563
A glycopolypeptide (gp 100) is the main antigen detected by HTLV-III antisera.
Med Microbiol Immunol. 1985;174(1):35-42 PMID: 2987654
Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS.
Science. 1984 May 4;224(4648):500-3 PMID: 6200936
The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus.
Nature. 1984 Dec 20-1985 Jan 2;312(5996):763-7 PMID: 6096719
Serological analysis of a subgroup of human T-lymphotropic retroviruses (HTLV-III) associated with AIDS.
Science. 1984 May 4;224(4648):503-5 PMID: 6200937
AIDS-associated retroviruses (ARV) can productively infect other cells besides human T helper cells.
Virology. 1985 Dec;147(2):441-8 PMID: 2416120
Elongation of DNA complementary to the 5' end of the avian sarcoma virus genome by the virion-associated RNA-dependent DNA polymerase.
J Virol. 1979 May;30(2):438-52 PMID: 89203
Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma.
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7415-9 PMID: 6261256
Selective tropism of lymphadenopathy associated virus (LAV) for helper-inducer T lymphocytes.
Science. 1984 Jul 6;225(4657):59-63 PMID: 6328660
Serologic identification and characterization of a macaque T-lymphotropic retrovirus closely related to HTLV-III.
Science. 1985 Jun 7;228(4704):1199-201 PMID: 3873705
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
Long-term cultures of HTLV-III--infected T cells: a model of cytopathology of T-cell depletion in AIDS.
Science. 1986 Feb 21;231(4740):850-3 PMID: 2418502
A dot-immunobinding assay for monoclonal and other antibodies.
Anal Biochem. 1982 Jan 1;119(1):142-7 PMID: 7072935
Selective cytotoxicity of AIDS virus infection towards HTLV-I-transformed cell lines.
Int J Cancer. 1985 Oct 15;36(4):445-51 PMID: 2995259
Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS).
Science. 1983 May 20;220(4599):868-71 PMID: 6189183
Isolation of T-cell tropic HTLV-III-like retrovirus from macaques.
Science. 1985 Jun 7;228(4704):1201-4 PMID: 3159089
Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma.
Int J Cancer. 1977 May 15;19(5):621-6 PMID: 68013
T4 positive human neoplastic cell lines susceptible to and permissive for HTLV-III.
Lancet. 1984 Dec 22;2(8417-8418):1472-3 PMID: 6151082
Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS.
Science. 1984 May 4;224(4648):497-500 PMID: 6200935

Article Info

Journal

Medical microbiology and immunology

Abbr.

Med Microbiol Immunol

ISSN

0300-8584

DOI

10.1007/BF00190533

Published

1987-00-00

Pages

273-80

Language

English

Region

Germany

NLM ID

0314524

Subset

External Links

PubMed source DOI: 10.1007/BF00190533 View journal details

Analysis Services

Efficient replication of HTLV-III and STLV-IIImac in human Jurkat cells.

Contact

WeChat Official Account