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PMID: 1729233 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Regulation of the Salmonella typhimurium metA gene by the metR protein and homocysteine.

Journal of bacteriology ·Vol. 174 ·No. 2 ·1992-01-00 ·Pages 390-7

Mares R, Urbanowski ML, Stauffer GV

Abstract

The DNA sequence of the Salmonella typhimurium metA control region is presented. S1 nuclease mapping was used to determine the transcription initiation site. By measuring beta-galactosidase levels in Escherichia coli strains lysogenized with lambda phage carrying a metA-lacZ gene fusion, the MetR protein was shown to activate the metA gene. Homocysteine, an intermediate in methionine biosynthesis, plays a negative role in the MetR-mediated activation mechanism. Gel mobility shift assays and DNase I protection experiments showed that the MetR protein binds to a DNA fragment carrying the metA control region and protects a 26-bp region beginning 9 bp upstream of the -35 promoter sequence.

Related Genes
metA
MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Cloning, Molecular DNA-Binding Proteins/genetics,physiology Gene Expression Regulation, Bacterial Genes, Bacterial Homocysteine/genetics,physiology Lac Operon Molecular Sequence Data Protein Binding Regulatory Sequences, Nucleic Acid Salmonella typhimurium/genetics Trans-Activators/genetics Transcription, Genetic
Chemicals
Bacterial Proteins DNA-Binding Proteins MetR protein, Bacteria Trans-Activators Homocysteine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mares R
Department of Microbiology, University of Iowa, Iowa City 52242.
Urbanowski M L
Stauffer G V
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28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.2.390-397.1992
Published
1992-01-00
Pages
390-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205729
Subset
IM
Grants
NIGMS NIH HHS · GM38912 · United States
Databases
GENBANK
M74188, M79367, M79368, M79369, M79370, M79371, M79372, M84973, M84974, M84980
External Links
PubMed source DOI: 10.1128/jb.174.2.390-397.1992 PMC Full Text View journal details
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