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PMID: 2543976 Published · ppublish English Journal Article

Methionine synthesis in Escherichia coli: effect of the MetR protein on metE and metH expression.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 12 ·1989-06-00 ·Pages 4407-11

Cai XY, Maxon ME, Redfield B, Glass R, Brot N, Weissbach H

Abstract

Studies by Urbanowski et al. [Urbanowski, M. L., Stauffer, L. T., Plamann, L. S. & Stauffer, G. V. (1987) J. Bacteriol. 169, 1391-1397] have identified a regulatory locus, called metR, required for the expression of the metE and metH genes. We recently purified the MetR protein from Escherichia coli and showed that it could stimulate the in vitro expression of the metE gene and autoregulate its own synthesis. In the present study, the purified MetR protein has been shown to stimulate the in vitro expression of the metH gene. Also, the in vitro synthesized MetE, MetH, and MetR proteins were shown to be biologically active. The transcription start sites for the metE and metR genes have been determined, and DNA footprinting experiments have identified regions in the metE-metR intergenic sequence that are protected by either the MetR or MetJ proteins.

MeSH Terms
Bacterial Proteins/genetics Base Sequence Escherichia coli/genetics,metabolism Genes Genes, Bacterial Genes, Regulator Kinetics Methionine/biosynthesis Molecular Sequence Data
Chemicals
Bacterial Proteins Methionine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cai X Y
Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
Maxon M E
Redfield B
Glass R
Brot N
Weissbach H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.12.4407
Published
1989-06-00
Pages
4407-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC287278
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.12.4407 PMC Full Text View journal details
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