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PMID: 16593564 Published · ppublish English Journal Article

Regulation of methionine synthesis in Escherichia coli: Effect of metJ gene product and S-adenosylmethionine on the expression of the metF gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 11 ·1985-06-00 ·Pages 3601-5

Shoeman R, Redfield B, Coleman T, Greene RC, Smith AA, Brot N, Weissbach H

Abstract

The regulation of the expression of the Escherichia coli metF gene, which codes for 5,10-methylenetet-rahydrofolate reductase (EC 1.1.99.15), has been investigated by using a simplified DNA-directed in vitro system that measures the formation of the first dipeptide (fMet-Ser) of the gene product. The synthesis of fMet-Ser directed by a plasmid containing the metF gene is specifically inhibited by metJ protein (repressor protein). S-Adenosylmethionine enhances the inhibition by the metJ protein of metF gene expression. The inhibition by the metJ protein is at the level of transcription and the results suggest that S-adenosylmethionine is functioning as an allosteric effector.

Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Shoeman R
Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
Redfield B
Coleman T
Greene R C
Smith A A
Brot N
Weissbach H
References (27)
27 references, click to expand
  1. DNA-directed in vitro synthesis of proteins involved in bacterial transcription and translation.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6115-9 PMID: 160561
  2. Regulation of the terminal reactions in methionine biosynthesis by vitamin B 12 and methionine.
    Arch Biochem Biophys. 1972 May;150(1):23-31 PMID: 4554945
  3. DNA-directed in vitro synthesis of beta-galactosidase. Studies with purified factors.
    J Biol Chem. 1977 Oct 10;252(19):6889-94 PMID: 561072
  4. Purification and properties of a soluble factor required for the deoxyribonucleic acid-directed in vitro synthesis of beta-galactosidase.
    J Biol Chem. 1975 Feb 25;250(4):1556-62 PMID: 1089661
  5. Methods for the analysis and preparation of adenosylmethionine and adenosylhomocysteine.
    Anal Biochem. 1966 May;15(2):323-33 PMID: 4289755
  6. Properties of metK mutants of Escherichia coli K-12.
    J Bacteriol. 1973 Jul;115(1):57-67 PMID: 4577753
  7. Regulation by methionine of the synthesis of a third aspartokinase and of a second homoserine dehydrogenase in Escherichia coli K 12.
    Biochim Biophys Acta. 1967 Mar 22;136(2):245-7 PMID: 4860558
  8. Effect of L-methionine and vitamin B 12 on methionine biosynthesis in Escherichia coli.
    Arch Biochem Biophys. 1969 Sep;133(2):413-9 PMID: 4898017
  9. S-Adenosylmethionine synthetase deficient mutants of Escherichia coli K-12 with impaired control of methionine biosynthesis.
    Biochem Biophys Res Commun. 1970 Mar 27;38(6):1120-6 PMID: 4908544
  10. Isolation and some properties of methionine transfer ribonucleic acid from Escherichia coli.
    Biochemistry. 1970 Apr 14;9(8):1694-700 PMID: 4909079
  11. Regulation of S-adenosylmethionine synthetase in Escherichia coli.
    J Bacteriol. 1970 Nov;104(2):734-47 PMID: 4923071
  12. Regulation of methionine biosynthesis in Escherichia coli: mapping of the metJ locus and properties of a metJ plus-metJ minus diploid.
    Proc Natl Acad Sci U S A. 1971 Feb;68(2):367-71 PMID: 5277087
  13. Resistance to norleucine and control of methionine synthesis in Escherichia coli.
    Nature. 1965 May 29;206(987):962-3 PMID: 5320374
  14. Methionyl soluble ribonucleic acid transformylase. I. Purification and partial characterization.
    J Biol Chem. 1967 Apr 10;242(7):1522-5 PMID: 5337045
  15. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  16. In vitro regulation of phage lambda cII gene expression by Escherichia coli integration host factor.
    Proc Natl Acad Sci U S A. 1984 Oct;81(19):6009-13 PMID: 6091131
  17. Structural studies of lambda transducing bacteriophage carrying bacterial deoxyribonucleic acid from the metBJLF region of the Escherichia coli chromosome.
    J Bacteriol. 1981 Aug;147(2):612-21 PMID: 6267016
  18. Translational control of the expression of the beta subunit gene of E. coli RNA polymerase.
    Biochem Biophys Res Commun. 1983 Jun 29;113(3):1018-25 PMID: 6307301
  19. A coupled DNA-directed in vitro system to study gene expression based on di- and tripeptide formation.
    Methods Enzymol. 1983;101:690-706 PMID: 6310342
  20. Nucleotide sequence of metF, the E. coli structural gene for 5-10 methylene tetrahydrofolate reductase and of its control region.
    Nucleic Acids Res. 1983 Oct 11;11(19):6723-32 PMID: 6356036
  21. In vitro expression of Escherichia coli ribosomal protein L 10 gene: tripeptide synthesis as a measure of functional mRNA.
    Arch Biochem Biophys. 1982 Oct 15;218(2):572-8 PMID: 6760818
  22. Use of different tRNASer isoacceptor species in vitro to discriminate between the expression of plasmid genes.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1466-8 PMID: 6803242
  23. Linkage map of Escherichia coli K-12, edition 6.
    Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
  24. Translational control of ribosomal protein L10 synthesis occurs prior to formation of first peptide bond.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4261-4 PMID: 7027258
  25. [The specific inhibiting effect of methionine in the formation of methionine-synthase by Escherichia coli].
    C R Hebd Seances Acad Sci. 1953 Feb 16;236(7):746-8 PMID: 13051887
  26. [Inhibition of the synthesis of the enzymes participating in the formation of tryptophan in Escherichia coli].
    C R Hebd Seances Acad Sci. 1959 Jun 15;248(24):3490-2 PMID: 13671770
  27. Expression of Escherichia coli ribosomal protein and RNA polymerase genes cloned on plasmids.
    Mol Gen Genet. 1979 May 23;173(1):39-50 PMID: 381841
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.11.3601
Published
1985-06-00
Pages
3601-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397833
External Links
PubMed source DOI: 10.1073/pnas.82.11.3601 PMC Full Text View journal details
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