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PMID: 3512521 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Autoregulation by tandem promoters of the Salmonella typhimurium LT2 metJ gene.

Journal of bacteriology ·Vol. 165 ·No. 3 ·1986-03-00 ·Pages 740-5

Urbanowski ML, Stauffer GV

Abstract

Regulation of the Salmonella typhimurium metJ gene was examined by measuring beta-galactosidase activity in Escherichia coli strains lysogenic for a phage carrying a metJ-lacZ gene fusion. The results indicated that the metJ gene is regulated by its own gene product and by methionine supplementation to the growth medium. This autoregulatory mechanism involved two tandem promoters, pJ1 and pJ2, separated by approximately 65 base pairs. Deletion analysis permitted the assessment of the activity of promoters pJ1 and pJ2 individually. Promoter Pj1 was negatively regulated by the metJ gene product and by methionine. Although Pj2 regulation remained unclear, evidence is presented which suggests that it is not negatively regulated like pJ1.

MeSH Terms
DNA, Recombinant Gene Expression Regulation Methionine/biosynthesis,pharmacology Promoter Regions, Genetic Repressor Proteins/genetics Salmonella typhimurium/genetics Transcription, Genetic
Chemicals
DNA, Recombinant Repressor Proteins Methionine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Urbanowski M L
Stauffer G V
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18 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.165.3.740-745.1986
Published
1986-03-00
Pages
740-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214491
Subset
IM
Grants
NIGMS NIH HHS · GM26878 · United States
External Links
PubMed source DOI: 10.1128/jb.165.3.740-745.1986 PMC Full Text View journal details
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