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PMID: 6330744 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

DNA sequence of the carA gene and the control region of carAB: tandem promoters, respectively controlled by arginine and the pyrimidines, regulate the synthesis of carbamoyl-phosphate synthetase in Escherichia coli K-12.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 13 ·1984-07-00 ·Pages 4134-8

Piette J, Nyunoya H, Lusty CJ, Cunin R, Weyens G, Crabeel M, Charlier D, Glansdorff N, Piérard A

Abstract

The carAB operon of Escherichia coli K-12, which encodes the two subunits of carbamoyl-phosphate synthetase (glutamine hydrolyzing) [carbon-dioxide: L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating); EC 6.3.5.5], is cumulatively repressed by arginine and the pyrimidines. We describe the structure of the control region of carAB and the sequence of the carA gene. Nuclease S1 mapping experiments show that two adjacent tandem promoters within the carAB control region serve as initiation sites. The upstream promoter P1 is controlled by pyrimidines; the downstream promoter P2 is regulated by arginine. Attenuation control does not appear to be involved in the expression of carAB. A possible mechanism by which control at these promoters concurs to produce a cumulative pattern of repression is discussed. The translational start of carA is atypical; it consists of a UUG or AUU codon.

MeSH Terms
Amino Acid Sequence Arginine/pharmacology Base Sequence Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)/biosynthesis,genetics Cloning, Molecular DNA Restriction Enzymes Enzyme Repression Escherichia coli/enzymology,genetics Genes/drug effects Genes, Bacterial/drug effects Ligases/genetics Operon/drug effects Pyrimidines/pharmacology Transcription, Genetic
Chemicals
Pyrimidines Arginine DNA Restriction Enzymes Ligases Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Piette J
Nyunoya H
Lusty C J
Cunin R
Weyens G
Crabeel M
Charlier D
Glansdorff N
Piérard A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.13.4134
Published
1984-07-00
Pages
4134-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345383
Subset
IM
Grants
NIGMS NIH HHS · GM 25846 · United States
Databases
GENBANK
J01597
External Links
PubMed source DOI: 10.1073/pnas.81.13.4134 PMC Full Text View journal details
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