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PMID: 1729227 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The Salmonella typhimurium virulence plasmid complement resistance gene rck is homologous to a family of virulence-related outer membrane protein genes, including pagC and ail.

Journal of bacteriology ·Vol. 174 ·No. 1 ·1992-01-00 ·Pages 84-91

Heffernan EJ, Harwood J, Fierer J, Guiney D

Abstract

A fragment of the Salmonella typhimurium virulence plasmid containing the rck locus, when cloned in the recombinant cosmid pADE016, was shown previously to confer high-level complement resistance on both rough and smooth Escherichia coli, Salmonella minnesota, and S. typhimurium and was associated with the production of an outer membrane protein. We determined the nucleotide sequence of the fragment containing the rck locus. Mutations in the two major open reading frames confirmed that the complement resistance mediated by pADE016 was due to a single 555-bp rck gene encoding a 17-kDa outer membrane protein. Analysis of the rck gene revealed that the Rck outer membrane protein consisted of 185 amino acid residues, with a calculated postcleavage molecular mass of 17.4 kDa. Rck is homologous to a family of outer membrane proteins expressed in gram-negative bacteria, two of which have been associated with virulence-related phenotypes: PagC, required by S. typhimurium for survival in macrophages and for virulence in mice; and Ail, a product of the Yersinia enterocolitica chromosome capable of mediating bacterial adherence to and invasion of epithelial cell lines. Rck, most closely related to PagC, represents the third outer membrane protein in this five-member family with a distinct virulence-associated phenotype.

Related Genes
pagC rck
MeSH Terms
Amino Acid Sequence Bacterial Outer Membrane Proteins/genetics Base Sequence Complement System Proteins/immunology DNA Mutational Analysis Molecular Sequence Data Phylogeny Plasmids/genetics Promoter Regions, Genetic Restriction Mapping Salmonella typhimurium/genetics,immunology,pathogenicity Sequence Homology, Nucleic Acid Transcription, Genetic Virulence/genetics
Chemicals
Bacterial Outer Membrane Proteins Complement System Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Heffernan E J
Department of Medicine, University of California, San Diego 92103.
Harwood J
Fierer J
Guiney D
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.1.84-91.1992
Published
1992-01-00
Pages
84-91
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205680
Subset
IM
Grants
NIADDK NIH HHS · AM35108 · United States
Databases
GENBANK
D13260, L01135, L01136, L01137, L01138, L01139, L01140, L01141, M76130, Z11768
External Links
PubMed source DOI: 10.1128/jb.174.1.84-91.1992 PMC Full Text View journal details
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