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PMID: 6092858 Published · ppublish English Comparative Study Journal Article

DNA sequence analysis of the Serratia marcescens ompA gene: implications for the organisation of an enterobacterial outer membrane protein.

Molecular & general genetics : MGG ·Vol. 195 ·No. 1-2 ·1984-00-00 ·Pages 321-8

Braun G, Cole ST

Abstract

The cloned ompA gene from Serratia marcescens was fully expressed in Escherichia coli and its product correctly assembled into the outer membrane. The S. marcescens polypeptide was not functionally equivalent to the E. coli OmpA protein, which serves as a phage receptor and as a component of several colicin uptake systems. DNA sequence analysis of the gene showed that three regions of the protein likely to be exposed on the cell surface not only differed extensively from the corresponding regions of the E. coli polypeptide but also from all other sequenced OmpA proteins. It is suggested that this sequence polymorphism represents a safety mechanism by which the various enterobacterial species can avoid cross-infection by noxious agents such as phages or colicins.

MeSH Terms
Bacterial Outer Membrane Proteins/genetics Base Sequence Cloning, Molecular Codon DNA Restriction Enzymes Enterobacteriaceae/genetics Escherichia coli/genetics Genes Genes, Bacterial Nucleic Acid Conformation RNA, Messenger/genetics Serratia marcescens/genetics Species Specificity
Chemicals
Bacterial Outer Membrane Proteins Codon RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Braun G
Cole S T
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53 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00332766
Published
1984-00-00
Pages
321-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X00618
External Links
PubMed source DOI: 10.1007/BF00332766
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