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PMID: 10872320 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The ultimate localization of an outer membrane protein of Escherichia coli K-12 is not determined by the signal sequence.

The EMBO journal ·Vol. 2 ·No. 8 ·1983-00-00 ·Pages 1275-9

Tommassen J, van Tol H, Lugtenberg B

Abstract

To study the role of the signal sequences in the biogenesis of outer membrane proteins, we have constructed two hybrid genes: a phoE-ompF hybrid gene, which encodes the signal sequence of outer membrane PhoE protein and the structural sequence of outer membrane OmpF protein, and a bla-phoE hybrid gene which encodes the signal sequence as well as 158 amino acids of the structural sequence of the periplasmic enzyme beta-lactamase and the complete structural sequence of PhoE protein. The products of these genes are normally transported to and assembled into the outer membrane These results show: (i) that signal sequences of exported proteins are export signals which function independently of the structural sequence, and (ii) that the information which determines the ultimate location of an outer membrane protein is located in the structural sequence of this protein, and not in the signal sequence.

MeSH Terms
Amino Acid Sequence Bacterial Outer Membrane Proteins/genetics,metabolism Bacterial Proteins/genetics,metabolism Base Sequence DNA, Bacterial Escherichia coli/genetics,metabolism Escherichia coli Proteins Molecular Sequence Data Porins/genetics,metabolism Protein Sorting Signals/genetics,metabolism
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins DNA, Bacterial Escherichia coli Proteins OmpF protein Porins Protein Sorting Signals PhoE protein, E coli
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tommassen J
Institute for Molecular Biology, State University, Utrecht, The Netherlands.
van Tol H
Lugtenberg B
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
1275-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555272
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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