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PMID: 17172357 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The coactivator function of Arabidopsis NPR1 requires the core of its BTB/POZ domain and the oxidation of C-terminal cysteines.

The Plant cell ·Vol. 18 ·No. 12 ·2006-12-00 ·Pages 3670-85

Rochon A, Boyle P, Wignes T, Fobert PR, Després C

Abstract

NONEXPRESSOR OF PATHOGENESIS-RELATED GENES1 (NPR1) regulates systemic acquired resistance (SAR) in Arabidopsis thaliana, and current models propose that after treatment with salicylic acid (SA), Cys-82 and Cys-216 of NPR1 are reduced, leading to nuclear import. The interaction of nucleus-localized NPR1 with TGA transcription factors results in the activation of defense genes, including the SAR marker PATHOGENESIS-RELATED-1 (PR-1), and the deployment of SAR. Little is known about how TGA factors or NPR1 regulate transcription or whether a TGA-NPR1 complex forms on DNA. We show that TGA2 and NPR1 are recruited to PR-1 independently of each other and of SA treatment. Consistent with the result that a triple knockout in TGA2/5/6 derepresses PR-1, in vivo plant transcription assays revealed that TGA2 is not an autonomous transcription activator but is a transcriptional repressor in both untreated and SA-treated cells. However, after stimulation with SA, TGA2 is incorporated into a transactivating complex with NPR1, forming an enhanceosome that requires the core of the NPR1 BTB/POZ domain (residues 80 to 91) and the oxidation of NPR1 Cys-521 and Cys-529. These Cys residues are found in a new type of transactivation domain that we term Cys-oxidized. These data further our understanding of the mechanism by which TGA2 and NPR1 activate Arabidopsis PR-1.

MeSH Terms
Amino Acid Sequence Arabidopsis/cytology,drug effects,genetics,metabolism Arabidopsis Proteins/chemistry,genetics,metabolism Basic-Leucine Zipper Transcription Factors/genetics,metabolism Cysteine/metabolism Gene Expression Regulation, Plant/drug effects Models, Genetic Molecular Sequence Data Nuclear Proteins/genetics,metabolism Oxidation-Reduction/drug effects Plant Proteins/genetics Promoter Regions, Genetic/drug effects,genetics Protein Structure, Tertiary/drug effects Protein Transport/drug effects RNA, Messenger/genetics,metabolism Salicylic Acid/pharmacology Structure-Activity Relationship Transcriptional Activation/drug effects
Chemicals
AHBP-1b protein, Arabidopsis Arabidopsis Proteins Basic-Leucine Zipper Transcription Factors NPR1 protein, Arabidopsis Nuclear Proteins Plant Proteins RNA, Messenger pathogenesis-related proteins, plant Cysteine Salicylic Acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rochon Amanda
Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada L2S 3A1.
Boyle Patrick
Wignes Tracy
Fobert Pierre R
Després Charles
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.106.046953
Published
2006-12-00
Epub
2006-00-15
Pages
3670-85
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1785396
Subset
IM
Databases
PDB
1BUO
External Links
PubMed source DOI: 10.1105/tpc.106.046953 PMC Full Text View journal details
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