Generation of broad-spectrum disease resistance by overexpression of an essential regulatory gene in systemic acquired resistance.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 11 ·1998-05-26 ·Pages 6531-6

Cao H, Li X, Dong X

Abstract

The recently cloned NPR1 gene of Arabidopsis thaliana is a key regulator of acquired resistance responses. Upon induction, NPR1 expression is elevated and the NPR1 protein is activated, in turn inducing expression of a battery of downstream pathogenesis-related genes. In this study, we found that NPR1 confers resistance to the pathogens Pseudomonas syringae and Peronospora parasitica in a dosage-dependent fashion. Overexpression of NPR1 leads to enhanced resistance with no obvious detrimental effect on the plants. Thus, for the first time, a single gene is shown to be a workable target for genetic engineering of nonspecific resistance in plants.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Cao H

Developmental, Cell, and Molecular Biology Group, Department of Botany, Box 91000, Duke University, Durham, NC 27708-1000, USA.

Li X

Dong X

References (31)

31 references, click to expand

Suppression and Restoration of Lesion Formation in Arabidopsis lsd Mutants.
Plant Cell. 1995 Dec;7(12):2013-2022 PMID: 12242366
Increased tolerance to two oomycete pathogens in transgenic tobacco expressing pathogenesis-related protein 1a.
Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7327-31 PMID: 8346252
Characterization of a salicylic acid-insensitive mutant (sai1) of Arabidopsis thaliana, identified in a selective screen utilizing the SA-inducible expression of the tms2 gene.
Mol Plant Microbe Interact. 1997 Jan;10(1):69-78 PMID: 9002272
The Arabidopsis NIM1 protein shows homology to the mammalian transcription factor inhibitor I kappa B.
Plant Cell. 1997 Mar;9(3):425-39 PMID: 9090885
A mutation in Arabidopsis that leads to constitutive expression of systemic acquired resistance.
Plant Cell. 1994 Dec;6(12):1845-57 PMID: 7866028
Arabidopsis mutants simulating disease resistance response.
Cell. 1994 May 20;77(4):565-77 PMID: 8187176
Arabidopsis signal transduction mutant defective in chemically and biologically induced disease resistance.
Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6602-6 PMID: 11607555
Molecular genetics of plant disease resistance.
Science. 1995 May 5;268(5211):661-7 PMID: 7732374
The cpr5 mutant of Arabidopsis expresses both NPR1-dependent and NPR1-independent resistance.
Plant Cell. 1997 Sep;9(9):1573-84 PMID: 9338960
Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat.
Plant Cell. 1996 Apr;8(4):629-43 PMID: 8624439
Resistance gene-dependent plant defense responses.
Plant Cell. 1996 Oct;8(10):1773-91 PMID: 8914325
How and Why Do Plants Inactivate Homologous (Trans)genes?
Plant Physiol. 1995 Mar;107(3):679-685 PMID: 12228391
Programmed cell death in plants: a pathogen-triggered response activated coordinately with multiple defense functions.
Cell. 1994 May 20;77(4):551-63 PMID: 8187175
Systemic acquired resistance induced by localized virus infections in plants.
Virology. 1961 Jul;14:340-58 PMID: 13743578
Requirement of salicylic Acid for the induction of systemic acquired resistance.
Science. 1993 Aug 6;261(5122):754-6 PMID: 17757215
The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats.
Cell. 1997 Jan 10;88(1):57-63 PMID: 9019406
Coordinate Gene Activity in Response to Agents That Induce Systemic Acquired Resistance.
Plant Cell. 1991 Oct;3(10):1085-1094 PMID: 12324583
Genetics and Utilization of Pathogen Resistance in Plants.
Plant Cell. 1996 Oct;8(10):1747-1755 PMID: 12239360
Isolation of Arabidopsis mutants with enhanced disease susceptibility by direct screening.
Genetics. 1996 Jun;143(2):973-82 PMID: 8725243
Enhanced quantitative resistance against fungal disease by combinatorial expression of different barley antifungal proteins in transgenic tobacco.
Plant J. 1995 Jul;8(1):97-109 PMID: 7655510
Plant Disease Resistance Genes: Function Meets Structure.
Plant Cell. 1996 Oct;8(10):1757-1771 PMID: 12239361
Acquired resistance in Arabidopsis.
Plant Cell. 1992 Jun;4(6):645-56 PMID: 1392589
Signals and transduction mechanisms for activation of plant defenses against microbial attack.
Cell. 1989 Jan 27;56(2):215-24 PMID: 2643475
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
Plant disease resistance genes in signal perception and transduction.
Cell. 1994 Feb 11;76(3):419-22 PMID: 8313464
Salicylic acid is a systemic signal and an inducer of pathogenesis-related proteins in virus-infected tobacco.
Plant Cell. 1991 Aug;3(8):809-18 PMID: 1820820
Characterization of an Arabidopsis Mutant That Is Nonresponsive to Inducers of Systemic Acquired Resistance.
Plant Cell. 1994 Nov;6(11):1583-1592 PMID: 12244227
Transgenic Plants with Enhanced Resistance to the Fungal Pathogen Rhizoctonia solani.
Science. 1991 Nov 22;254(5035):1194-7 PMID: 17776411
Gene-for-gene complementarity in plant-pathogen interactions.
Annu Rev Genet. 1990;24:447-63 PMID: 2088175
Systemic Acquired Resistance.
Plant Cell. 1996 Oct;8(10):1809-1819 PMID: 12239363
Osmotin overexpression in potato delays development of disease symptoms.
Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1888-92 PMID: 11607463

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.95.11.6531

Published

1998-05-26

Pages

6531-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC34547

External Links

PubMed source DOI: 10.1073/pnas.95.11.6531 PMC Full Text View journal details

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Generation of broad-spectrum disease resistance by overexpression of an essential regulatory gene in systemic acquired resistance.

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