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PMID: 10662863 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Arabidopsis NPR1/NIM1 protein enhances the DNA binding activity of a subgroup of the TGA family of bZIP transcription factors.

The Plant cell ·Vol. 12 ·No. 2 ·2000-02-00 ·Pages 279-90

Després C, DeLong C, Glaze S, Liu E, Fobert PR

Abstract

The Arabidopsis NPR1 gene is essential in activating systemic, inducible plant defense responses. To gain a better understanding of NPR1 function, we conducted a yeast two-hybrid screening procedure and identified a differential interaction between NPR1 and all known members of the Arabidopsis TGA family of basic leucine zipper transcription factors. In the electrophoretic mobility shift assay, NPR1 substantially increased the binding of TGA2 to its cognate promoter element (as-1) as well as to a positive salicylic acid-inducible element (LS7) and a negative element (LS5) in the promoter of the pathogenesis-related PR-1 gene. Proteins encoded by npr1 mutants interacted poorly with TGA2 and did not substantially increase TGA2 binding to the as-1, LS5, or LS7 elements, thus establishing a link between the loss of disease resistance and the loss of TGA2 interaction and NPR1-enhanced DNA binding. Coupled with observations that the DNA binding activity of TGA factors is deregulated in npr1 plants, the results suggest that NPR1-mediated DNA binding of TGA2 is critical for activation of defense genes.

MeSH Terms
Arabidopsis/genetics,metabolism Arabidopsis Proteins Base Sequence Cell Nucleus/metabolism DNA Primers DNA-Binding Proteins/metabolism Fungal Proteins/metabolism Plant Proteins/metabolism Promoter Regions, Genetic Protein Kinases Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae Proteins Transcription Factors/metabolism Two-Hybrid System Techniques
Chemicals
Arabidopsis Proteins DNA Primers DNA-Binding Proteins Fungal Proteins NPR1 protein, Arabidopsis Plant Proteins Saccharomyces cerevisiae Proteins Transcription Factors NPR1 protein, S cerevisiae Protein Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Després C
National Research Council Canada, Plant Biotechnology Institute, 110 Gymnasium Place, Saskatoon, Saskatchewan, Canada S7N 0W9.
DeLong C
Glaze S
Liu E
Fobert P R
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
Published
2000-02-00
Pages
279-90
Language
English
Region
England
NLM ID
9208688
PMCID
PMC139764
Subset
IM
Corrections
CommentIn
PMID: 10662855
External Links
PubMed source PMC Full Text View journal details
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