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PMID: 11148282 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nuclear localization of NPR1 is required for activation of PR gene expression.

The Plant cell ·Vol. 12 ·No. 12 ·2000-12-00 ·Pages 2339-2350

Kinkema M, Fan W, Dong X

Abstract

Systemic acquired resistance (SAR) is a broad-spectrum resistance in plants that involves the upregulation of a battery of pathogenesis-related (PR) genes. NPR1 is a key regulator in the signal transduction pathway that leads to SAR. Mutations in NPR1 result in a failure to induce PR genes in systemic tissues and a heightened susceptibility to pathogen infection, whereas overexpression of the NPR1 protein leads to increased induction of the PR genes and enhanced disease resistance. We analyzed the subcellular localization of NPR1 to gain insight into the mechanism by which this protein regulates SAR. An NPR1-green fluorescent protein fusion protein, which functions the same as the endogenous NPR1 protein, was shown to accumulate in the nucleus in response to activators of SAR. To control the nuclear transport of NPR1, we made a fusion of NPR1 with the glucocorticoid receptor hormone binding domain. Using this steroid-inducible system, we clearly demonstrate that nuclear localization of NPR1 is essential for its activity in inducing PR genes.

MeSH Terms
Arabidopsis Proteins Base Sequence Cell Nucleus/metabolism DNA Primers Fungal Proteins/genetics,metabolism Gene Expression Regulation, Plant Green Fluorescent Proteins Luminescent Proteins/genetics Nuclear Localization Signals Plant Proteins/genetics Protein Kinases Recombinant Fusion Proteins/genetics,metabolism Saccharomyces cerevisiae Proteins
Chemicals
Arabidopsis Proteins DNA Primers Fungal Proteins Luminescent Proteins Nuclear Localization Signals Plant Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins proline-rich protein, Arabidopsis Green Fluorescent Proteins NPR1 protein, S cerevisiae Protein Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kinkema M
Developmental, Cell, and Molecular Biology Group, Department of Biology, Duke University, Durham, North Carolina 27708-1000, USA.
Fan W
Dong X
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.12.12.2339
Published
2000-12-00
Pages
2339-2350
Language
English
Region
England
NLM ID
9208688
PMCID
PMC102222
Subset
IM
Grants
NIGMS NIH HHS · F32 GM019498 · United States
NIGMS NIH HHS · F32-GM19498 · United States
External Links
PubMed source DOI: 10.1105/tpc.12.12.2339 PMC Full Text View journal details
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