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PMID: 7780308 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Small cysteine-rich antifungal proteins from radish: their role in host defense.

The Plant cell ·Vol. 7 ·No. 5 ·1995-05-00 ·Pages 573-88

Terras FR, Eggermont K, Kovaleva V, Raikhel NV, Osborn RW, Kester A, Rees SB, Torrekens S, Van Leuven F, Vanderleyden J

Abstract

Radish seeds have previously been shown to contain two homologous, 5-kD cysteine-rich proteins designated Raphanus sativus-antifungal protein 1 (Rs-AFP1) and Rs-AFP2, both of which exhibit potent antifungal activity in vitro. We now demonstrate that these proteins are located in the cell wall and occur predominantly in the outer cell layers lining different seed organs. Moreover, Rs-AFPs are preferentially released during seed germination after disruption of the seed coat. The amount of released proteins is sufficient to create a microenvironment around the seed in which fungal growth is suppressed. Both the cDNAs and the intron-containing genomic regions encoding the Rs-AFP preproteins were cloned. Transcripts (0.55 kb) hybridizing with an Rs-AFP1 cDNA-derived probe were present in near-mature and mature seeds. Such transcripts as well as the corresponding proteins were barely detectable in healthy uninfected leaves but accumulated systemically at high levels after localized fungal infection. The induced leaf proteins (designated Rs-AFP3 and Rs-AFP4) were purified and shown to be homologous to seed Rs-AFPs and to exert similar antifungal activity in vitro. A chimeric Rs-AFP2 gene under the control of the constitutive cauliflower mosaic virus 35S promoter conferred enhanced resistance to the foliar pathogen Alternaria longipes in transgenic tobacco. The term "plant defensins" is proposed to denote these defense-related proteins.

MeSH Terms
Alternaria/pathogenicity Amino Acid Sequence Antifungal Agents Antimicrobial Cationic Peptides Base Sequence Cloning, Molecular Defensins Fusarium/pathogenicity Immunity, Innate/genetics Immunohistochemistry Microbial Sensitivity Tests Molecular Sequence Data Plant Diseases Plant Proteins/genetics,isolation & purification,physiology Plants, Genetically Modified Plants, Toxic Seeds/chemistry,genetics,physiology Sequence Homology, Amino Acid Tobacco/genetics Vegetables/chemistry,genetics,physiology
Chemicals
Antifungal Agents Antimicrobial Cationic Peptides Defensins Plant Proteins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Terras F R
F.A. Janssens Laboratory of Genetics, Katholieke Universiteit Leuven, Heverlee, Belgium.
Eggermont K
Kovaleva V
Raikhel N V
Osborn R W
Kester A
Rees S B
Torrekens S
Van Leuven F
Vanderleyden J
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.7.5.573
Published
1995-05-00
Pages
573-88
Language
English
Region
England
NLM ID
9208688
PMCID
PMC160805
Subset
IM
Databases
GENBANK
U18556, U18557
External Links
PubMed source DOI: 10.1105/tpc.7.5.573 PMC Full Text View journal details
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