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PMID: 171627 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequences spanning the EcoRI substrate site.

Nucleic acids research ·Vol. 2 ·No. 10 ·1975-10-00 ·Pages 1851-65

Garfin DE, Boyer HW, Goodman HM

Abstract

Substrate recognition by the EcoRI restriction endonuclease was investigated by analysis of the nucleotide sequences at the sites of enzymatic cleavage in various DNA molecules. 5'-end labeling and homochromatographic fingerprinting led to the determination of a 17-base-pair sequence spanning the EcoRI site of simian virus 40 DNA and a 15-base-pair sequence overlapping the EcoRI site of Col El plasmid DNA. Three other DNAs were similarly tested, although extended sequences were not determined in these cases. The EcoRI site was shown to be symmetric double-stranded equivalent of -N-G-A-A-T-T-C-N-.

MeSH Terms
Base Sequence Binding Sites DNA/analysis DNA Restriction Enzymes Deoxyribonucleases Endonucleases Escherichia coli/enzymology Methods Oligonucleotides/analysis Phosphoric Diester Hydrolases Protein Binding
Chemicals
Oligonucleotides DNA Deoxyribonucleases Endonucleases DNA Restriction Enzymes Phosphoric Diester Hydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Garfin D E
Boyer H W
Goodman H M
References (28)
28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/2.10.1851
Published
1975-10-00
Pages
1851-65
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343552
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/2.10.1851 PMC Full Text View journal details
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