Nucleotide sequence analysis with polynucleotide kinase and nucleotide "mapping" methods. 5'-Terminal sequences of deoxyribonucleic acid from bacteriophages lambda and 424.

The Biochemical journal ·Vol. 131 ·No. 3 ·1973-03-00 ·Pages 569-82

Murray K

Abstract

The polynucleotide kinase reaction was used in analyses of complex mixtures of oligodeoxynucleotides which were fractionated by various two-dimensional nucleotide ;mapping' procedures. Parallel ionophoretic analyses on DEAE-cellulose paper, pH2, and AE-cellulose paper, pH3.5, of venom phosphodiesterase partial digests of 5'-terminally labelled oligonucleotides enabled the sequence of the nucleotides to be deduced uniquely. A ;diagonal ionophoresis' method has been used with mixtures of nucleotides. Application of these methods to 5'-terminally labelled DNA from bacteriophage lambda gave the terminal sequences pA-G-G-T-C-G and pG-G-G-C-G. Identical 5'-terminal sequences were found with DNA from bacteriophage 424.

MeSH Terms

Adenine Nucleotides/analysis Adenosine Triphosphate Animals Autoradiography Base Sequence Coliphages/analysis Cytosine Nucleotides/analysis DNA, Viral/analysis Deoxyribonucleases Deoxyribonucleotides/analysis Electrophoresis, Paper Endonucleases Escherichia coli/enzymology Guanine Nucleotides/analysis Hydrogen-Ion Concentration Mutation Oligonucleotides/analysis Pancreas/enzymology Phosphoric Diester Hydrolases Phosphorus Isotopes Phosphotransferases/analysis Polynucleotides Snakes Species Specificity Thymine Nucleotides/analysis Venoms

Chemicals

Adenine Nucleotides Cytosine Nucleotides DNA, Viral Deoxyribonucleotides Guanine Nucleotides Oligonucleotides Phosphorus Isotopes Polynucleotides Thymine Nucleotides Venoms Adenosine Triphosphate Phosphotransferases Deoxyribonucleases Endonucleases Phosphoric Diester Hydrolases

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Murray K

References (25)

25 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1310569

Published

1973-03-00

Pages

569-82

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1177504

Subset

External Links

PubMed source DOI: 10.1042/bj1310569 PMC Full Text View journal details

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Nucleotide sequence analysis with polynucleotide kinase and nucleotide "mapping" methods. 5'-Terminal sequences of deoxyribonucleic acid from bacteriophages lambda and 424.

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