The nucleotide sequence preceding an RNA polymerase initiation site on SV40 DNA. Part 1. The sequence of the late strand transcript.

Nucleic acids research ·Vol. 1 ·No. 4 ·1974-04-00 ·Pages 577-94

Zain BS, Weissman SM, Dhar R, Pan J

Abstract

The nucleotide sequence of the transcript of the "late" strand of the region of SV40 DNA preceding the preferred initiation site for Escherichia coli RNA polymerase has been determined to be U-G-U-A-A-C-C-A-U-U-A-U-A-A-G-C-U-G-C-A-A-U-A-A-A-C-A-A-G-U-U-A-A-C-A-A-C-A-A-C-A-A-U-U-G-Cp. Hemophilus influenza restriction endonuclease cleaves this region 30 nucleotides (base pairs) before the site of initiation of RNA synthesis by RNA polymerase.

MeSH Terms

Base Sequence Binding Sites/genetics DNA Restriction Enzymes/metabolism DNA, Viral/genetics,metabolism DNA-Directed RNA Polymerases/metabolism Escherichia coli/enzymology Haemophilus influenzae/enzymology Molecular Sequence Data RNA, Complementary/genetics,isolation & purification RNA, Viral/genetics,isolation & purification Simian virus 40/genetics,metabolism Transcription, Genetic

Chemicals

DNA, Viral RNA, Complementary RNA, Viral DNA-Directed RNA Polymerases DNA Restriction Enzymes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Zain B S

Weissman S M

Dhar R

Pan J

References (15)

15 references, click to expand

Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose.
Eur J Biochem. 1969 Dec;11(2):395-9 PMID: 4902612
[THE PROMOTOR, A GENETIC ELEMENT NECESSARY TO THE EXPRESSION OF AN OPERON].
C R Hebd Seances Acad Sci. 1964 Mar 16;258:3125-8 PMID: 14143651
A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.
J Mol Biol. 1970 Jul 28;51(2):379-91 PMID: 5312500
A restriction enzyme from Hemophilus influenzae. II.
J Mol Biol. 1970 Jul 28;51(2):393-409 PMID: 5312501
Nucleotide sequence of a ribonucleic acid transcribed in vitro from lambda phage deoxyribonucleic acid.
J Biol Chem. 1971 Aug 25;246(16):5120-39 PMID: 4936723
RNA synthesis startpoints in bacteriophage lambda: are the promoter and operator transcribed?
Nat New Biol. 1972 Jun 21;237(77):227-32 PMID: 4556643
In vitro transcription.
Annu Rev Biochem. 1972;41:409-46 PMID: 4628438
Bidirectional replication of Simian Virus 40 DNA.
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3097-100 PMID: 4343955
Analysis of simian virus 40 DNA with the restriction enzyme of Haemophilus aegyptius, endonuclease Z.
J Virol. 1973 Apr;11(4):508-14 PMID: 4349491
Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. V. Isolation of additional hybrids which differ in their simian virus 40-specific biological properties.
J Virol. 1973 May;11(5):655-64 PMID: 4350710
Preferred site for initiation of RNA transcription by Escherichia coli RNA polymerase within the simian virus 40 DNA segment of the nondefective adenovirus-simian virus 40 hybrid viruses Ad2 + ND 1 and Ad2 + ND 3 .
J Virol. 1973 May;11(5):682-93 PMID: 4350713
Preferred sites of digestion of a ribonuclease from Enterobacter sp. in the sequence analysis of Bacillus stearothermophilus 5S ribonucleic acid.
Biochemistry. 1973 Jul 17;12(15):2901-4 PMID: 4719125
Recognition sequence of a restriction enzyme.
Nat New Biol. 1973 Jul 4;244(131):7-10 PMID: 4578426
Structure of the lambda operators.
Nature. 1973 Nov 16;246(5429):133-6 PMID: 4586104
SV40 DNA strand selection by Escherichia coli RNA polymerase.
J Mol Biol. 1970 Jun 14;50(2):407-20 PMID: 4320049

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/1.4.577

Published

1974-04-00

Pages

577-94

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343358

Subset

External Links

PubMed source DOI: 10.1093/nar/1.4.577 PMC Full Text View journal details

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The nucleotide sequence preceding an RNA polymerase initiation site on SV40 DNA. Part 1. The sequence of the late strand transcript.

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