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PMID: 16649111 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The heat stress transcription factor HsfA2 serves as a regulatory amplifier of a subset of genes in the heat stress response in Arabidopsis.

Plant molecular biology ·Vol. 60 ·No. 5 ·2006-03-00 ·Pages 759-72

Schramm F, Ganguli A, Kiehlmann E, Englich G, Walch D, von Koskull-Döring P

Abstract

Within the Arabidopsis family of 21 heat stress transcription factors (Hsfs) HsfA2 is the strongest expressed member under heat stress (hs) conditions. Irrespective of the tissue, HsfA2 accumulates under heat stress similarly to other heat stress proteins (Hsps). A SALK T-DNA insertion line with a complete HsfA2-knockout was analyzed with respect to the changes in the transcriptome under heat stress conditions. Ascorbate peroxidase 2 (APX2) was identified as the most affected transcript in addition to several sHsps, individual members of the Hsp70 and Hsp100 family, as well as many transcripts of genes with yet unknown functions. For functional validation, the transcription activation potential of HsfA2 on GUS reporter constructs containing 1 kb upstream promoter sequences of selected target genes were analyzed using transient reporter assays in mesophyll protoplasts. By deletion analysis the promoter region of the strongest affected target gene APX2 was functionally mapped in detail to verify potential HsfA2 binding sites. By electrophoretic mobility shift assays we identified TATA-Box proximal clusters of heat stress elements (HSE) in the promoters of selected target genes as potential HsfA2 binding sites. The results presented here demonstrate that the expression of HsfA2 in Arabidopsis is strictly heat stress-dependent and this transcription factor represents a regulator of a subset of stress response genes in Arabidopsis.

MeSH Terms
Arabidopsis/genetics,metabolism Arabidopsis Proteins/genetics,metabolism Binding Sites/genetics Blotting, Western DNA-Binding Proteins/genetics,metabolism Electrophoretic Mobility Shift Assay Gene Expression Regulation, Plant Glucuronidase/genetics,metabolism Heat Shock Transcription Factors Heat-Shock Proteins/genetics,metabolism Hot Temperature Mutation Peroxidases/genetics,metabolism Plant Proteins/genetics,metabolism Promoter Regions, Genetic/genetics Protoplasts/metabolism Recombinant Fusion Proteins/genetics,metabolism Reverse Transcriptase Polymerase Chain Reaction Tobacco/genetics,metabolism Transcription Factors/genetics,metabolism Transfection
Chemicals
Arabidopsis Proteins DNA-Binding Proteins HSFA2 protein, Arabidopsis Heat Shock Transcription Factors Heat-Shock Proteins Plant Proteins Recombinant Fusion Proteins Transcription Factors Peroxidases Glucuronidase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Schramm Franziska
Institute of Molecular Bio Sciences, Biocenter N200/R306, Goethe University, Marie-Curie-Str. 9, D-60439, Frankfurt, Germany.
Ganguli Arnab
Kiehlmann Elke
Englich Gisela
Walch Daniela
von Koskull-Döring Pascal
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/s11103-005-5750-x
Published
2006-03-00
Pages
759-72
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/s11103-005-5750-x View journal details
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