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PMID: 11599559 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need?

Cell stress & chaperones ·Vol. 6 ·No. 3 ·2001-07-00 ·Pages 177-89

Nover L, Bharti K, Döring P, Mishra SK, Ganguli A, Scharf KD

Abstract

Sequencing of the Arabidopsis genome revealed a unique complexity of the plant heat stress transcription factor (Hsf) family. By structural characteristics and phylogenetic comparison, the 21 representatives are assigned to 3 classes and 14 groups. Particularly striking is the finding of a new class of Hsfs (AtHsfC1) closely related to Hsf1 from rice and to Hsfs identified from frequently found expressed sequence tags of tomato, potato, barley, and soybean. Evidently, this new type of Hsf is well expressed in different plant tissues. Besides the DNA binding and oligomerization domains (HR-A/B region), we identified other functional modules of Arabidopsis Hsfs by sequence comparison with the well-characterized tomato Hsfs. These are putative motifs for nuclear import and export and transcriptional activation (AHA motifs). There is intriguing flexibility of size and sequence in certain parts of the otherwise strongly conserved N-terminal half of these Hsfs. We have speculated about possible exon-intron borders in this region in the ancient precursor gene of plant Hsfs, similar to the exon-intron structure of the present mammalian Hsf-encoding genes.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics,metabolism Arabidopsis Proteins/chemistry,genetics,metabolism DNA-Binding Proteins/chemistry,genetics,metabolism Heat Shock Transcription Factors Heat Stress Disorders Heat-Shock Proteins/chemistry,genetics,metabolism Lycopersicon esculentum/genetics,metabolism Molecular Sequence Data Phylogeny Plant Proteins Protein Structure, Tertiary Sequence Alignment Transcription Factors/chemistry,genetics,metabolism
Chemicals
Arabidopsis Proteins DNA-Binding Proteins HSF1 protein, Arabidopsis Heat Shock Transcription Factors Heat-Shock Proteins Plant Proteins Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Nover L
Biocenter of the Goethe University, Frankfurt/Main, Germany. nover@cellbiology.uni-frankfurt.de
Bharti K
Döring P
Mishra S K
Ganguli A
Scharf K D
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Article Info
Journal
Cell stress & chaperones
Abbr.
Cell Stress Chaperones
ISSN
1355-8145
DOI
10.1379/1466-1268(2001)006<0177:aathst>2.0.co;2
Email
nover@cellbiology.uni-frankfurt.de
Published
2001-07-00
Pages
177-89
Language
English
Region
Netherlands
NLM ID
9610925
PMCID
PMC434399
Subset
IM
External Links
PubMed source DOI: 10.1379/1466-1268(2001)006<0177:aathst>2.0.co;2 PMC Full Text View journal details
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