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PMID: 1654434 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The human DNA-activated protein kinase phosphorylates simian virus 40 T antigen at amino- and carboxy-terminal sites.

Journal of virology ·Vol. 65 ·No. 10 ·1991-10-00 ·Pages 5131-40

Chen YR, Lees-Miller SP, Tegtmeyer P, Anderson CW

Abstract

Protein phosphorylation modulates the functions of simian virus 40 large T antigen (TAg) in productive and transforming infections. We recently described a DNA-activated protein kinase (DNA-PK) that efficiently phosphorylates TAg and several other nuclear, DNA-binding proteins in vitro (S.P. Lees-Miller, Y.-R. Chen, and C. W. Anderson, Mol. Cell. Biol. 10:6472-6481, 1990). In this report, we show by direct amino acid sequence analysis that DNA-PK phosphorylates TAg strongly at Ser-677, a residue known to be important for TAg interaction with origin site I and for transformation. We propose that DNA-PK may modulate the role of TAg in repressing early viral transcription and cell transformation, but a role for DNA-PK in regulating simian virus 40 DNA synthesis is not excluded. DNA-PK also phosphorylates Ser-665, and Ser-667, and one or more serines between amino acids 110 and 131. At least six serines, Ser-111, Ser-112, Ser-120, Ser-665, Ser-667, and Ser-677, are phosphorylated in TAg purified from baculovirus vector-infected insect cells.

MeSH Terms
Amino Acid Sequence Animals Antigens, Polyomavirus Transforming/genetics,isolation & purification,metabolism Baculoviridae/genetics Cell Line Chromatography, High Pressure Liquid DNA/metabolism Genetic Vectors Humans Insecta Molecular Sequence Data Peptide Fragments/isolation & purification Phosphates/metabolism Phosphopeptides/isolation & purification Phosphorylation Protein Kinases/metabolism Recombinant Proteins/isolation & purification,metabolism Simian virus 40/immunology,metabolism
Chemicals
Antigens, Polyomavirus Transforming Peptide Fragments Phosphates Phosphopeptides Recombinant Proteins DNA Protein Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chen Y R
Biology Department, Brookhaven National Laboratory, Upton, New York 11973.
Lees-Miller S P
Tegtmeyer P
Anderson C W
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.10.5131-5140.1991
Published
1991-10-00
Pages
5131-40
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC248989
Subset
IM
Grants
NIAID NIH HHS · AI25170 · United States
NCI NIH HHS · CA18808 · United States
NCI NIH HHS · CA28146 · United States
External Links
PubMed source DOI: 10.1128/JVI.65.10.5131-5140.1991 PMC Full Text View journal details
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