Home LiteratureArticle Details
PMID: 6175963 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Simian virus 40 large tumor antigen: a "RNA binding protein"?

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 4 ·1982-02-00 ·Pages 1139-43

Khandjian EW, Loche M, Darlix JL, Cramer R, Türler H, Weil R

Abstract

Simian virus 40 large tumor antigen was isolated by immunoaffinity chromatography from monkey or mouse cell cultures undergoing lytic or transforming infection. RNase-treated gel-purified large tumor antigen, on hydrolysis with alkali, gave about equimolar amounts of AMP, GMP, CMP, and UMP. Furthermore, RNA fragments of approximately 45 nucleotides could be isolated from large tumor antigen purified by the same procedure. Mapping of the T1 oligonucleotides showed a high complexity, as indicated by the presence of unique sequences of 15-30 nucleotides and of poly(A). This is compatible with the hypothesis that these RNA fragments are derived from cellular pre-mRNAs or mRNAs. Our results suggest that Simian virus 40 large tumor antigen is a RNA-binding protein and might possibly be involved in regulation of synthesis, maturation, or translation of cellular mRNAs.

MeSH Terms
Antigens, Neoplasm/analysis,isolation & purification Antigens, Viral/analysis,isolation & purification Antigens, Viral, Tumor Carrier Proteins/analysis Oligoribonucleotides/analysis RNA/analysis RNA, Messenger/metabolism RNA-Binding Proteins Simian virus 40/immunology
Chemicals
Antigens, Neoplasm Antigens, Viral Antigens, Viral, Tumor Carrier Proteins Oligoribonucleotides RNA, Messenger RNA-Binding Proteins RNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Khandjian E W
Loche M
Darlix J L
Cramer R
Türler H
Weil R
References (32)
32 references, click to expand
  1. SV40 virus-induced tumour specific transplantation antigen in cultured mouse cells.
    Nature. 1970 Jul 11;227(5254):141-5 PMID: 4316952
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. Preparative two-dimensional polyacrylamide gel electrophoresis of 32 P-labeled RNA.
    Anal Biochem. 1972 Sep;49(1):184-97 PMID: 5081097
  4. Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon.
    J Virol. 1972 Oct;10(4):591-8 PMID: 4343541
  5. Gel electrophoresis of RNA under denaturing conditions.
    Biochim Biophys Acta. 1973 Oct 26;324(3):320-33 PMID: 4202717
  6. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  7. T antigen binds to simian virus 40 DNA at the origin of replication.
    Proc Natl Acad Sci U S A. 1975 Apr;72(4):1605-9 PMID: 165517
  8. A simplifying concept in tumor virology: virus-specific "pleiotropic effectors".
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:381-95 PMID: 169084
  9. Mapping the genes of simian virus 40.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:53-60 PMID: 169090
  10. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  11. Two forms of simian-virus-40-specific T-antigen in abortive and lytic infection.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1097-101 PMID: 177974
  12. Chromosomal proteins: tightly bound nucleic acid and its bearing on the measurement of nonhistone protein phosphorylation.
    Anal Biochem. 1976 Apr;71(2):393-404 PMID: 179446
  13. Identification of regions of the SV40 genome which contain preferred SV40 T antigen-binding sites.
    Cell. 1976 Aug;8(4):535-45 PMID: 182383
  14. Modification of simian virus 40 protein A.
    J Virol. 1977 Feb;21(2):647-57 PMID: 189092
  15. The binding site on SV40 DNA for a T antigen-related protein.
    Cell. 1978 Jan;13(1):165-79 PMID: 202398
  16. Organization and expression of early genes of simian virus 40.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):117-21 PMID: 203926
  17. Complete nucleotide sequence of SV40 DNA.
    Nature. 1978 May 11;273(5658):113-20 PMID: 205802
  18. The genome of simian virus 40.
    Science. 1978 May 5;200(4341):494-502 PMID: 205947
  19. Viral 'tumor antigens': A novel type of mammalian regulator protein.
    Biochim Biophys Acta. 1978 Nov 17;516(3):301-88 PMID: 215209
  20. Effect of alkylation on the physical properties of simian virus 40 T-antigen species.
    J Virol. 1979 Feb;29(2):587-96 PMID: 219248
  21. Phosphorylation of T-antigen and control T-antigen expression in cells transformed by wild-type and tsA mutants of simian virus 40.
    J Virol. 1979 Feb;29(2):753-62 PMID: 219256
  22. Simian virus 40 early mRNA's. I. Genomic localization of 3' and 5' termini and two major splices in mRNA from transformed and lytically infected cells.
    J Virol. 1979 Apr;30(1):279-96 PMID: 90157
  23. Simian virus 40 large T antigen isoelectric focuses as multiple species with varying phosphate content.
    Virology. 1979 Dec;99(2):413-6 PMID: 229628
  24. Specific association of simian virus 40 tumor antigen with simian virus 40 chromatin.
    J Virol. 1980 Jan;33(1):78-87 PMID: 6245256
  25. Simian virus 40 and polyoma virus stimulate overall cellular RNA and protein synthesis.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1476-80 PMID: 6154935
  26. Amino acid sequence analysis of fragments generated by partial proteolysis from large simian virus 40 tumor antigen.
    J Biol Chem. 1980 Jun 25;255(12):5627-34 PMID: 6247340
  27. Charge isomers of simian virus 40 T-antigen.
    FEBS Lett. 1980 Sep 8;118(2):229-32 PMID: 6252053
  28. Phosphorylation of SV40 large T antigen.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:165-9 PMID: 6253130
  29. T antigens of SV40-transformed cells.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:171-8 PMID: 6253131
  30. Modification of SV40 T antigen by poly ADP-ribosylation.
    Cell. 1981 May;24(2):567-72 PMID: 7237559
  31. Binding of a simian virus 40 T antigen-related protein to DNA.
    J Mol Biol. 1981 Jan 25;145(3):471-88 PMID: 6267291
  32. THE NATURE AND LOCALIZATION OF THE SV 40-INDUCED COMPLEMENT-FIXING ANTIGEN.
    Proc Natl Acad Sci U S A. 1965 Mar;53:684-92 PMID: 14338251
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.4.1139
Published
1982-02-00
Pages
1139-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345916
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.4.1139 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2