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PMID: 1641341 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence and variability of the 5.8S and 26S rRNA genes of Pneumocystis carinii.

Nucleic acids research ·Vol. 20 ·No. 14 ·1992-07-25 ·Pages 3763-72

Liu Y, Rocourt M, Pan S, Liu C, Leibowitz MJ

Abstract

The sequence of the coding region of the rRNA operon of rat-derived Pneumocystis carinii has been completed, including the genes for 5.8S and 26S rRNA. These genes show homology to the rRNA genes of yeast, and an apparent group I self-splicing intron is present in the 26S rRNA gene. Like a similar intron in the 16S rRNA gene, this intron is in a phylogenetically conserved region. Variation in the 26S rRNA sequence was noted between P. carinii organisms isolated from two different sources.

MeSH Terms
Base Sequence Cloning, Molecular Genes, Fungal Introns/genetics Molecular Sequence Data Nucleic Acid Conformation Operon/genetics Pneumocystis/genetics Polymerase Chain Reaction Polymorphism, Genetic/genetics RNA, Catalytic/genetics RNA, Fungal/genetics RNA, Ribosomal/genetics RNA, Ribosomal, 5.8S/genetics Sequence Homology, Nucleic Acid
Chemicals
RNA, Catalytic RNA, Fungal RNA, Ribosomal RNA, Ribosomal, 5.8S RNA, ribosomal, 26S
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Liu Y
Department of Molecular Genetics and Microbiology, UMDNJ--Robert Wood Johnson Medical School, Piscataway 08854-5635.
Rocourt M
Pan S
Liu C
Leibowitz M J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.14.3763
Published
1992-07-25
Pages
3763-72
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334029
Subset
IM
Databases
GENBANK
J01359, J04683, K00471, K01051, M10752, M13612, M35013, M86759, M86760, M86761, V00192, X02447, X05682, X06574, X17454
External Links
PubMed source DOI: 10.1093/nar/20.14.3763 PMC Full Text View journal details
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