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PMID: 2160429 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of Pneumocystis carinii chromosomes and mapping of five genes.

Infection and immunity ·Vol. 58 ·No. 6 ·1990-06-00 ·Pages 1705-10

Lundgren B, Cotton R, Lundgren JD, Edman JC, Kovacs JA

Abstract

Pulsed field gel electrophoresis was used to identify the chromosome-size DNA of Pneumocystis carinii, a major pathogen of immunocompromised patients. Thirteen chromosomes of rodent Pneumocystis carinii, ranging in size from 300 to 700 kilobases (kb), were identified. The minimum genome size for P. carinii, estimated on the basis of the sizes of chromosomes, is 7,000 kb. Genetic heterogeneity among different P. carinii isolates was documented by demonstration of chromosomal size variability. By hybridization studies, the genes for topoisomerase I, dihydrofolate reductase, rRNA, actin, and thymidylate synthase were mapped to single chromosomes of approximately 650, 590, 550, 460, and 350 kb, respectively. Hybridization studies further confirmed the genetic heterogeneity of P. carinii.

MeSH Terms
Actins/genetics Blotting, Southern Chromosome Mapping DNA Topoisomerases, Type I/genetics DNA, Ribosomal/genetics Electrophoresis, Agar Gel/methods Genes, Fungal Pneumocystis/genetics RNA, Ribosomal, 18S/genetics Restriction Mapping Tetrahydrofolate Dehydrogenase/genetics Thymidylate Synthase/genetics
Chemicals
Actins DNA, Ribosomal RNA, Ribosomal, 18S Tetrahydrofolate Dehydrogenase Thymidylate Synthase DNA Topoisomerases, Type I
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lundgren B
Critical Care Medicine, National Institutes of Health, National Institute of Alcohol Abuse and Alcoholism, Bethesda, Maryland 20892.
Cotton R
Lundgren J D
Edman J C
Kovacs J A
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.6.1705-1710.1990
Published
1990-06-00
Pages
1705-10
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258712
Subset
IM
Grants
NIAID NIH HHS · AI 27128 · United States
External Links
PubMed source DOI: 10.1128/iai.58.6.1705-1710.1990 PMC Full Text View journal details
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