Home LiteratureArticle Details
PMID: 2788266 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A self-splicing intron in the small subunit rRNA gene of Pneumocystis carinii.

Nucleic acids research ·Vol. 17 ·No. 13 ·1989-07-11 ·Pages 5349-59

Sogin ML, Edman JC

Abstract

The coding region for the Pneumocystis carinii small subunit rRNA (16S-like rRNA) contains 390 extra base pairs in a region that is highly conserved in other eukaryotic rRNA genes. The sequence has all the elements characteristic of group I self-splicing introns and interrupts the phylogenetically conserved helix proximal to the 3' terminus of the 16S-like rRNA. In vitro transcripts containing the putative group I intron were capable of undergoing a "self-splicing" reaction which required the presence of guanine nucleotide.

MeSH Terms
Animals Base Sequence Genes Introns Molecular Sequence Data Nucleic Acid Conformation Pneumocystis/genetics RNA Splicing RNA, Ribosomal/genetics RNA, Ribosomal, 16S/genetics
Chemicals
RNA, Ribosomal RNA, Ribosomal, 16S
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sogin M L
Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
Edman J C
References (22)
22 references, click to expand
  1. Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties.
    Nucleic Acids Res. 1988 Aug 11;16(15):7583-600 PMID: 2970625
  2. Ribosomal RNA sequence shows Pneumocystis carinii to be a member of the fungi.
    Nature. 1988 Aug 11;334(6182):519-22 PMID: 2970013
  3. Conservation of RNA secondary structures in two intron families including mitochondrial-, chloroplast- and nuclear-encoded members.
    EMBO J. 1983;2(1):33-8 PMID: 11894905
  4. Why genes in pieces?
    Nature. 1978 Feb 9;271(5645):501 PMID: 622185
  5. An insert in the single gene for the large ribosomal RNA in yeast mitochondrial DNA.
    Nature. 1978 Sep 28;275(5678):336-8 PMID: 357991
  6. Anatomy of the chloroplast ribosomal DNA of Chlamydomonas reinhardii.
    Cell. 1978 Oct;15(2):661-70 PMID: 719757
  7. An intervening sequence in the gene coding for 25S ribosomal RNA of Tetrahymena pigmentosa.
    Cell. 1979 Mar;16(3):565-73 PMID: 455443
  8. The gene for the 26 S rRNA in Physarum contains two insertions.
    FEBS Lett. 1979 Apr 15;100(2):347-50 PMID: 456573
  9. The intervening sequence in the 26S rRNA coding region of T. thermophila is transcribed within the largest stable precursor for rRNA.
    Cell. 1979 Oct;18(2):525-32 PMID: 498282
  10. The 10 kb Drosophila virilis 28S rDNA intervening sequence is flanked by a direct repeat of 14 base pairs of coding sequence.
    Nucleic Acids Res. 1980 Aug 25;8(16):3491-504 PMID: 6253902
  11. Nucleotide sequence of a ribosomal RNA gene intron from slime mold Physarum polycephalum.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1376-80 PMID: 6262791
  12. Comparison of fungal mitochondrial introns reveals extensive homologies in RNA secondary structure.
    Biochimie. 1982 Oct;64(10):867-81 PMID: 6817818
  13. Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena.
    Cell. 1982 Nov;31(1):147-57 PMID: 6297745
  14. The ribosomal RNA genes of Tetrahymena: structure and function.
    Eur J Cell Biol. 1985 Jan;36(1):133-51 PMID: 3884336
  15. Biological catalysis by RNA.
    Annu Rev Biochem. 1986;55:599-629 PMID: 2427016
  16. Length variation in eukaryotic rRNAs: small subunit rRNAs from the protists Acanthamoeba castellanii and Euglena gracilis.
    Gene. 1986;44(1):63-70 PMID: 3095190
  17. Phylogenetic evidence for the acquisition of ribosomal RNA introns subsequent to the divergence of some of the major Tetrahymena groups.
    EMBO J. 1986 Dec 20;5(13):3625-30 PMID: 3830129
  18. Structural diversity of eukaryotic small subunit ribosomal RNAs. Evolutionary implications.
    Ann N Y Acad Sci. 1987;503:125-39 PMID: 3304074
  19. Three-dimensional model of the active site of the self-splicing rRNA precursor of Tetrahymena.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):8788-92 PMID: 3480510
  20. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
    Methods Enzymol. 1987;155:335-50 PMID: 3431465
  21. The small-subunit ribosomal RNA gene sequences from the hypotrichous ciliates Oxytricha nova and Stylonychia pustulata.
    Mol Biol Evol. 1985 Sep;2(5):399-410 PMID: 3939705
  22. The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions.
    Gene. 1988 Nov 30;71(2):491-9 PMID: 3224833
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.13.5349
Published
1989-07-11
Pages
5349-59
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318115
Subset
IM
Grants
NIGMS NIH HHS · GM32964 · United States
Databases
GENBANK
X13687
External Links
PubMed source DOI: 10.1093/nar/17.13.5349 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2