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PMID: 3480510 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Three-dimensional model of the active site of the self-splicing rRNA precursor of Tetrahymena.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 24 ·1987-12-00 ·Pages 8788-92

Kim SH, Cech TR

Abstract

The rRNA intervening sequence of Tetrahymena is a catalytic RNA molecule, or "ribozyme." A tertiary-structure model of the active site of this ribozyme has been constructed based on comparative sequence analysis of related group I intervening sequences, data on the accessibility of each nucleotide to chemical and enzymatic probes, and principles of RNA folding derived from a consideration of the structure of tRNA determined by x-ray crystallography. In the model, the catalytic center has a two-helix structural framework composed of the base-paired segments of the group I conserved sequence elements. The structural framework supports and orients the conserved nucleotides that are adjacent to the base-paired sequence elements; these conserved nucleotides are proposed to form the active site and to bind the 5' splice-site duplex and the guanine nucleotide substrate. Tests of the model are proposed.

MeSH Terms
Animals Binding Sites Hydrogen Bonding Models, Molecular Nucleic Acid Conformation Nucleic Acid Precursors RNA Splicing RNA, Ribosomal Tetrahymena
Chemicals
Nucleic Acid Precursors RNA, Ribosomal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kim S H
Department of Chemistry, University of California, Berkeley 94720.
Cech T R
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33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.24.8788
Published
1987-12-00
Pages
8788-92
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299635
Subset
IM
Grants
NIGMS NIH HHS · GM28039 · United States
NIGMS NIH HHS · GM29287 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.24.8788 PMC Full Text View journal details
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