Examination of Genetic Relatedness of Marine Synechococcus spp. by Using Restriction Fragment Length Polymorphisms.

Applied and environmental microbiology ·Vol. 54 ·No. 12 ·1988-12-00 ·Pages 3071-8

Douglas SE, Carr N

Abstract

The relatedness of several marine Synechococcus spp. was estimated by DNA hybridization. Strains isolated from various geographical locations and representing a diversity of DNA base compositions and phycobiliprotein profiles were compared by restriction fragment length polymorphisms for a number of genes. DNAs from two marine red algae and a cryptomonad alga (which exhibit a phycobiliprotein composition similar to that of the marine Synechococcus spp.) and Synechococcus strain PCC6301 (Anacystis nidulans) were also included in the comparison. Strains WH8008, WH8018, and WH7805 were shown to be very similar to one another, as were strains WH7802 and WH7803. Strains WH8110 and WH5701 were clearly unrelated to any of the other strains, and no marine Synechococcus isolate showed any similarity to the freshwater Synechococcus strain PCC6301 or the eucaryotic algae. The method is relatively straightforward and sensitive and uses a variety of basic molecular biology techniques. Its utility in ascertaining the genetic relatedness and diversity of marine Synechococcus spp. and possible extension to field studies are discussed.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Douglas S E

National Research Council, 1411 Oxford Street, Halifax, Nova Scotia, Canada B3H 3ZI, and Department of Biological Sciences, University of Warwick, Coventry, England, CV4 7AL.

Carr N

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.54.12.3071-3078.1988

Published

1988-12-00

Pages

3071-8

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC204429

External Links

PubMed source DOI: 10.1128/aem.54.12.3071-3078.1988 PMC Full Text View journal details

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Examination of Genetic Relatedness of Marine Synechococcus spp. by Using Restriction Fragment Length Polymorphisms.

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