The use of NaOH as transfer solution of DNA onto nylon membrane decreases the hybridization efficiency.

Nucleic acids research ·Vol. 15 ·No. 2 ·1987-01-26 ·Pages 857

Rigaud G, Grange T, Pictet R

Abstract

暂无摘要

MeSH Terms

DNA/genetics Genetic Engineering/methods Nucleic Acid Hybridization Nylons Sodium Hydroxide Solutions

Chemicals

Nylons Solutions Sodium Hydroxide DNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Rigaud G

Grange T

Pictet R

References (2)

2 references, click to expand

Rapid transfer of DNA from agarose gels to nylon membranes.
Nucleic Acids Res. 1985 Oct 25;13(20):7207-21 PMID: 4059056
The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper.
Anal Biochem. 1980 Nov 15;109(1):123-9 PMID: 6162401

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

Published

1987-01-26

Pages

857

Language

English

Region

England

NLM ID

0411011

PMCID

PMC340476

Subset

External Links

Analysis Services