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PMID: 16592916 Published · ppublish English Journal Article

Nucleotide sequence of a cyanobacterial nifH gene coding for nitrogenase reductase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 11 ·1980-11-00 ·Pages 6476-80

Mevarech M, Rice D, Haselkorn R

Abstract

The nucleotide sequence of nifH, the structural gene for nitrogenase reductase (component II or Fe protein of nitrogenase) from the cyanobacterium Anabaena 7120 has been determined. Also reported are 194 bases of the 5'-flanking sequence and 170 bases of the 3'-flanking sequence. The predicted amino acid sequence was compared with that determined for the complete nitrogenase reductase of Clostridium pasteurianum and the cysteine-containing peptides of the protein from Azotobacter vinelandii. Amino acid sequences around five cysteines, located in the NH(2)-terminal two-thirds of the protein, are highly conserved in all three species. Codon usage in the first gene from a cyanobacterium to be sequenced shows striking asymmetries for eight amino acids.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mevarech M
Department of Biophysics and Theoretical Biology, The University of Chicago, Chicago, Illinois 60637.
Rice D
Haselkorn R
References (20)
20 references, click to expand
  1. Identification of blue-green algal nitrogen fixation genes by using heterologous DNA hybridization probes.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):186-90 PMID: 16592748
  2. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  3. Comparison of the iron proteins from the nitrogen fixation complexes of Azotobacter vinelandii, Clostridium pasteurianum, and Klebsiella pneumoniae.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3826-30 PMID: 7001444
  4. Interspecies homology of nitrogenase genes.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):191-5 PMID: 6987649
  5. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  6. Structure and function of nitrogenase.
    Annu Rev Biochem. 1979;48:387-418 PMID: 224803
  7. Interaction of the nitrogenase components of Anabaena cylindrica with those of Clostridium pasteurianum.
    Biochem Biophys Res Commun. 1978 Mar 30;81(2):280-7 PMID: 96817
  8. Molybdenum independence of nitrogenase component synthesis in the non-heterocystous cyanobacterium Plectonema.
    J Bacteriol. 1978 May;134(2):597-605 PMID: 96092
  9. Regulation and characterization of protein products coded by the nif (nitrogen fixation) genes of Klebsiella pneumoniae.
    J Bacteriol. 1978 Oct;136(1):267-79 PMID: 361694
  10. Fine-structure mapping and complementation analysis of nif (nitrogen fixation) genes in Klebsiella pneumoniae.
    J Bacteriol. 1978 Oct;136(1):253-66 PMID: 361693
  11. Genetic and biochemical analysis of mutants induced by bacteriophage Mu DNA integration into Klebsiella pneumoniae nitrogen fixation genes.
    Mol Gen Genet. 1978 Oct 4;165(2):181-9 PMID: 366377
  12. Complementation analysis of Klebsiella pneumoniae mutants defective in nitrogen fixation.
    Mol Gen Genet. 1977 Nov 29;157(2):189-98 PMID: 340923
  13. The amino acid sequence of Clostridium pasteurianum iron protein, a component of nitrogenase. III. The NH2-terminal and COOH-terminal sequences, tryptic peptides of large cyanogen bromide peptides, and the complete sequence.
    J Biol Chem. 1977 Oct 25;252(20):7093-100 PMID: 561783
  14. Sequence organization of a cloned tDNA met fragment from Xenopus laevis.
    Cell. 1978 Jul;14(3):713-24 PMID: 688390
  15. Complementary functioning of the component proteins of nitrogenase from several bacteria.
    J Bacteriol. 1978 Jun;134(3):936-43 PMID: 659370
  16. A simple method for DNA restriction site mapping.
    Nucleic Acids Res. 1976 Sep;3(9):2387-98 PMID: 787937
  17. Nitrogenase of Klebsiella pneumoniae. Purification and properties of the component proteins.
    Biochem J. 1972 Jul;128(3):655-75 PMID: 4344006
  18. Differentiation in Nostoc muscorum: nitrogenase is synthesized in heterocysts.
    Proc Natl Acad Sci U S A. 1973 Oct;70(10):2727-31 PMID: 4200722
  19. Structure of the molybdoferredoxin complex from Clostridium pasteurianum and isolation of its subunits.
    J Bacteriol. 1973 Feb;113(2):884-90 PMID: 4690968
  20. Molecular weights of nitrogenase components from Azotobacter vinelandii.
    Biochem Biophys Res Commun. 1975 Oct 27;66(4):1476-82 PMID: 1191303
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.11.6476
Published
1980-11-00
Pages
6476-80
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350308
External Links
PubMed source DOI: 10.1073/pnas.77.11.6476 PMC Full Text View journal details
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