Identification of blue-green algal nitrogen fixation genes by using heterologous DNA hybridization probes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 1 ·1980-01-00 ·Pages 186-90

Mazur BJ, Rice D, Haselkorn R

Abstract

In the filamentous blue-green alga Anabaena 7120, aerobic nitrogen fixation is linked to the differentiation of specialized cells called heterocysts. In order to study control of heterocyst development and nitrogen fixation in Anabaena, we have used cloned fragments of the Klebsiella pneumoniae nitrogen fixation (nif) genes as probes in DNA.DNA hybridizations with restriction endonuclease fragments of Anabaena DNA. Using this technique, we were able to identify and clone Anabaena nif genes, demonstrating the feasibility of using heterologous probes to identify genes for which no traditional genetic selection exists. From the patterns of hybridization observed, we deduced that although DNA sequence homology has been retained between some of the nif genes of these divergent organisms, the nif gene order has been rearranged.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Mazur B J

Department of Biophysics and Theoretical Biology, The University of Chicago, Chicago, Illinois 60637.

Rice D

Haselkorn R

References (27)

27 references, click to expand

PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT.
Biochim Biophys Acta. 1963 Aug 20;72:619-29 PMID: 14071565
Interspecies homology of nitrogenase genes.
Proc Natl Acad Sci U S A. 1980 Jan;77(1):191-5 PMID: 6987649
Cloning nitrogen fixing genes from Klebsiella pneumoniae in vitro and the isolation of NIF promoter mutants affecting glutamine synthetase regulation.
Basic Life Sci. 1977;9:111-28 PMID: 21649
Survey of extrachromosomal DNA found in the filamentous cyanobacteria.
J Bacteriol. 1978 Oct;136(1):414-8 PMID: 101520
Interaction of the nitrogenase components of Anabaena cylindrica with those of Clostridium pasteurianum.
Biochem Biophys Res Commun. 1978 Mar 30;81(2):280-7 PMID: 96817
Molybdenum independence of nitrogenase component synthesis in the non-heterocystous cyanobacterium Plectonema.
J Bacteriol. 1978 May;134(2):597-605 PMID: 96092
Isolation and preliminary characterization of auxotrophs of a filamentous Cyanobacterium.
J Bacteriol. 1977 Mar;129(3):1556-62 PMID: 403180
Overlapping sequences of Klebsiella pneumoniae nifDNA cloned and characterized.
Mol Gen Genet. 1979 Jul 2;174(1):59-66 PMID: 384162
Physical map of chromosomal nitrogen fixation (nif) genes of Klebsiella pneumoniae.
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2866-70 PMID: 379866
Regulation and characterization of protein products coded by the nif (nitrogen fixation) genes of Klebsiella pneumoniae.
J Bacteriol. 1978 Oct;136(1):267-79 PMID: 361694
Fine-structure mapping and complementation analysis of nif (nitrogen fixation) genes in Klebsiella pneumoniae.
J Bacteriol. 1978 Oct;136(1):253-66 PMID: 361693
Genetic and biochemical analysis of mutants induced by bacteriophage Mu DNA integration into Klebsiella pneumoniae nitrogen fixation genes.
Mol Gen Genet. 1978 Oct 4;165(2):181-9 PMID: 366377
Complementation analysis of Klebsiella pneumoniae mutants defective in nitrogen fixation.
Mol Gen Genet. 1977 Nov 29;157(2):189-98 PMID: 340923
Recombinant plasmid that carries part of the nitrogen fixation (nif) gene cluster of Klebsiella pneumoniae.
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2963-7 PMID: 331321
Heterotrophic micro- and macrocultures of a nitrogen-fixing cyanobacterium.
Arch Microbiol. 1976 Nov 2;110(23):145-7 PMID: 828022
Nucleotide sequence of the rightward operator of phage lambda.
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
Glutamine synthetase and ammonium regulation of nitrogenase synthesis in Klebsiella.
Nature. 1974 Oct 11;251(5475):481-5 PMID: 4153588
Regulation of nitrogen fixation in Klebsiella pneumoniae: evidence for a role of glutamine synthetase as a regulator of nitrogenase synthesis.
J Bacteriol. 1974 Nov;120(2):815-21 PMID: 4156359
In vitro synthesis of bacteriophage f1 proteins.
J Mol Biol. 1974 Feb 25;83(2):231-51 PMID: 4595423
Regulation of coliphage f1 single-stranded DNA synthesis by a DNA-binding protein.
J Mol Biol. 1973 Aug 5;78(2):285-300 PMID: 4747632
Nitrogenase activity in the blue-green alga Plectonema boryanum strain 594.
Arch Mikrobiol. 1970;73(3):250-60 PMID: 4992219
Is the heterocyst the site of nitrogen fixation in blue-green algae?
Nature. 1968 Nov 23;220(5169):810-2 PMID: 4880705
Mutants of Anabaena cylindrica altered in heterocyst spacing.
Arch Microbiol. 1975 May 5;103(3):219-23 PMID: 807173
Biochemistry and genetics of Klebsiella pneumoniae mutant strains unable to fix N2.
J Bacteriol. 1975 Mar;121(3):759-65 PMID: 1090602
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Physiological basis of the pattern of vegetative growth of a blue-green alga.
Proc Natl Acad Sci U S A. 1967 May;57(5):1246-51 PMID: 5231730
A membrane-filter technique for the detection of complementary DNA.
Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.77.1.186

Published

1980-01-00

Pages

186-90

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC348233

External Links

PubMed source DOI: 10.1073/pnas.77.1.186 PMC Full Text View journal details

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Identification of blue-green algal nitrogen fixation genes by using heterologous DNA hybridization probes.

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