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PMID: 384162 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Overlapping sequences of Klebsiella pneumoniae nifDNA cloned and characterized.

Molecular & general genetics : MGG ·Vol. 174 ·No. 1 ·1979-07-02 ·Pages 59-66

Cannon FC, Riedel GE, Ausubel FM

Abstract

A HindIII (17.0 kb) and an EcoR1 restriction fragment (6.9 kb) of Klebsiella pneumoniae nif DNA were cloned on two small amplifiable plasmids, pCM1 and pSA30 respectively. These plasmids between them carry 14 of the 15 known Klebsiella nif genes. The operon for the three structural genes for nitrogenase, nifpHDK, is carried on pSA30: four and five of the remaining six operons are on pCRA37 and pCM1 respectively. All of the nif genes were assigned to endonuclease restriction fragments of DNA using the Southern blotting technique (Southern, 1975) with total DNA of nif insertion mutants and radioactive plasmid DNA which contained cloned nif DNA sequences. Their locations were consistent with the genetic map of nif genes. The estimated size of the nif gene cluster was 24 kb.

MeSH Terms
Base Sequence DNA, Bacterial/analysis,genetics DNA, Recombinant/analysis Genes Klebsiella pneumoniae/genetics Nitrogenase/genetics Plasmids
Chemicals
DNA, Bacterial DNA, Recombinant Nitrogenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cannon F C
Riedel G E
Ausubel F M
References (17)
17 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00433306
Published
1979-07-02
Pages
59-66
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00433306
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