Home LiteratureArticle Details
PMID: 331321 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Recombinant plasmid that carries part of the nitrogen fixation (nif) gene cluster of Klebsiella pneumoniae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 7 ·1977-07-00 ·Pages 2963-7

Cannon FC, Riedel GE, Ausubel FM

Abstract

We have cloned fragments of the Klebsiella pneumoniae genome that carry part of the his operon and part of the nitrogen fixation (nif) gene cluster on the amplifiable plasmid pMB9. One particular plasmid, pCRA37, complements mutations in the hisD, nifB, and nifF loci. The physical map of pCRA37 as determined by restriction enzyme analysis correlates with the genetic map of the his-nif region as determined previously by phage P1-mediated cotransductional analysis.

MeSH Terms
Chromosome Mapping DNA Restriction Enzymes/metabolism DNA, Bacterial/metabolism DNA, Recombinant/metabolism Genes Genetic Linkage Histidine/metabolism Klebsiella pneumoniae Nitrogen Fixation Operon Plasmids
Chemicals
DNA, Bacterial DNA, Recombinant Histidine DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cannon F C
Riedel G E
Ausubel F M
References (28)
28 references, click to expand
  1. Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures.
    J Mol Biol. 1975 Jul 25;96(1):171-84 PMID: 169355
  2. Construction of a P plasmid carrying nitrogen fixation genes from Klebsiella pneumoniae.
    Nature. 1976 Mar 18;260(5548):268-71 PMID: 768778
  3. Expression of Klebsiella nitrogen fixation genes (nif) in Azotobacter.
    Nature. 1976 Mar 18;260(5548):271-2 PMID: 768779
  4. Derivation and properties of F-prime factors in Escherichia coli carrying nitrogen fixation genes from Klebsiella pneumoniae.
    J Gen Microbiol. 1976 Mar;93(1):111-25 PMID: 772161
  5. Use of bacteriophage Mu to isolate deletions in the his-nif region of Klebsiella pneumoniae.
    J Bacteriol. 1976 Dec;128(3):749-53 PMID: 791929
  6. Recombination between bacterial plasmids leading to the formation of plasmid multimers.
    Cell. 1976 Dec;9(4 PT 2):707-16 PMID: 797459
  7. Protection of particular cleavage sites of restriction endonucleases by distamycin A and actinomycin D.
    Nucleic Acids Res. 1976 Sep;3(9):2293-301 PMID: 967694
  8. Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit.
    Cell. 1976 Nov;9(3):431-8 PMID: 1033038
  9. Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.
    J Bacteriol. 1975 Jan;121(1):354-62 PMID: 1090574
  10. Biochemistry and genetics of Klebsiella pneumoniae mutant strains unable to fix N2.
    J Bacteriol. 1975 Mar;121(3):759-65 PMID: 1090602
  11. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
    J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
  12. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  13. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
    J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
  14. Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase.
    Biochemistry. 1973 Dec 4;12(25):5045-50 PMID: 4366077
  15. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  16. The nitrogen fixation genes.
    Nature. 1972 Oct 27;239(5374):495-9 PMID: 4563018
  17. Construction of biologically functional bacterial plasmids in vitro.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4 PMID: 4594039
  18. Chromosomal integration of Klebsiella nitrogen fixation genes in Escherichia coli.
    J Gen Microbiol. 1974 Jan;80(1):227-39 PMID: 4595005
  19. Plasmids formed in nitrogen-fixing Escherichia coli-Klebsiella pneumoniae hybrids.
    J Gen Microbiol. 1974 Jan;80(1):241-51 PMID: 4595006
  20. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455-9 PMID: 4610576
  21. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  22. Genetic analysis of the histidine operon in Escherichia coli K12.
    Genetics. 1970 Oct;66(2):219-29 PMID: 4934197
  23. Histidine-requiring mutants of Escherichia coli K12.
    Genetics. 1970 Oct;66(2):231-44 PMID: 4934198
  24. Transfer of nitrogen-fixation genes by conjugation in Klebsiella pneumoniae.
    Nature. 1971 Nov 5;234(5323):47-8 PMID: 4942884
  25. The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis.
    J Mol Biol. 1972 Feb 14;63(3):383-95 PMID: 5014925
  26. Transduction of the nitrogen-fixation genes in Klebsiella pneumoniae.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1174-7 PMID: 5288365
  27. A model for three-point analysis of random general transduction.
    Genetics. 1966 Aug;54(2):405-10 PMID: 5338813
  28. Endonuclease recognition sites mapped on Zea mays chloroplast DNA.
    Proc Natl Acad Sci U S A. 1976 Dec;73(12):4309-13 PMID: 16592373
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.7.2963
Published
1977-07-00
Pages
2963-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431364
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.7.2963 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2