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PMID: 6096868 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genes for the alpha and beta subunits of phycocyanin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 24 ·1984-12-00 ·Pages 7946-50

de Lorimier R, Bryant DA, Porter RD, Liu WY, Jay E, Stevens SE

Abstract

Phycocyanin (PC) is a light-harvesting protein common to blue-green and red algae. We have isolated the genes for the two apoprotein subunits, alpha and beta, of PC from the blue-green alga Agmenellum quadruplicatum PR-6. We synthesized eight sense-strand tetradecameric oligonucleotide probes that could encode a particular pentapeptide in PC alpha from A. quadruplicatum. Only one probe hybridized with total RNA from this organism. This oligonucleotide showed homology to a unique restriction fragment when used to probe Southern blots of A. quadruplicatum DNA. The probe-homologous 3.1-kilobase pair HindIII fragment was cloned. The nucleotide sequence of a 1.7-kilobase pair segment of this clone was determined. Two open reading frames are contained in this region, which correspond in deduced amino acid sequence to PC alpha and PC beta subunits. Both coding sequences are in the same orientation, separated by 105 base pairs, with the PC beta gene 5' to the PC alpha gene. Each gene has a Shine-Dalgarno-type sequence near the initiation codon. Codon frequencies in the two genes may be correlated with the abundance of their products. The deduced amino acid sequences of the gene products show considerable homology with the alpha and beta PC subunits from other species.

MeSH Terms
Amino Acid Sequence Base Sequence Codon Cyanobacteria/genetics DNA Restriction Enzymes Genes Macromolecular Substances Nucleic Acid Hybridization Phycocyanin/genetics Pigments, Biological/genetics
Chemicals
Codon Macromolecular Substances Pigments, Biological Phycocyanin DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
de Lorimier R
Bryant D A
Porter R D
Liu W Y
Jay E
Stevens S E
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.24.7946
Published
1984-12-00
Pages
7946-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392270
Subset
IM
Grants
NIGMS NIH HHS · GM28609 · United States
NIGMS NIH HHS · GM31625 · United States
Databases
GENBANK
K02660
External Links
PubMed source DOI: 10.1073/pnas.81.24.7946 PMC Full Text View journal details
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