Transformation in Agmenellum quadruplicatum.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 10 ·1980-10-00 ·Pages 6052-6

Stevens SE, Porter RD

Abstract

A DNA-mediated transformation system for the blue-green alga Agmenellum quadruplicatum, strain PR-6, is described and characterized for DNA concentration dependence, dependence on time of exposure to DNA, phenotypic expression, sensitivity to various enzymes, and competence. The stability of the transformants has been investigated, and genetic backcross and selfing experiments have been performed. This system fulfills all of the criteria established for the well-characterized transformation systems in heterotrophic bacteria and demonstrates significant similarities to at least one of these systems for all characteristics examined. The efficiency of transformation is high. This system fills a need for a well-characterized genetic system in an oxygen-evolving photoautotroph. We have used it to transform a strain with a mutational lesion in assimilatory nitrogen metabolism to a wild-type genotype.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Stevens S E

Department of Microbiology, Cell Biology, Biochemistry, and Biophysics, The Pennsylvania State University, University Park, Pennsylvania 16802.

Porter R D

References (18)

18 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.77.10.6052

Published

1980-10-00

Pages

6052-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC350211

External Links

PubMed source DOI: 10.1073/pnas.77.10.6052 PMC Full Text View journal details

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