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PMID: 6193726 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

A rapid method for detecting specific RNA transcripts by hybridization to DNA probes in solution.

Analytical biochemistry ·Vol. 131 ·No. 1 ·1983-05-00 ·Pages 141-5

Nobrega FG, Dieckmann CL, Tzagoloff A

Abstract

A method is described for detecting specific transcripts in crude mixtures of RNA. The method employs hybridization of single-stranded or double-stranded radioactive DNA probes in solution, followed by electrophoretic separation of the hybrid and probe on agarose and visualization by radioautography. The procedure offers the advantages of decreased preparation time and increased sensitivity over currently used methods.

MeSH Terms

Autoradiography Base Composition Base Sequence Cytochrome b Group/genetics DNA/analysis DNA, Single-Stranded Electrophoresis, Agar Gel Nucleic Acid Hybridization RNA/isolation & purification Saccharomyces cerevisiae

Chemicals

Cytochrome b Group DNA, Single-Stranded RNA DNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Nobrega F G

Dieckmann C L

Tzagoloff A

Article Info

Journal

Analytical biochemistry

Abbr.

Anal Biochem

ISSN

0003-2697

DOI

10.1016/0003-2697(83)90145-8

Published

1983-05-00

Pages

141-5

Language

English

Region

United States

NLM ID

0370535

Subset

External Links

PubMed source DOI: 10.1016/0003-2697(83)90145-8 View journal details

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A rapid method for detecting specific RNA transcripts by hybridization to DNA probes in solution.

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