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PMID: 16047201 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Structural and functional analysis of 5S rRNA in Saccharomyces cerevisiae.

Molecular genetics and genomics : MGG ·Vol. 274 ·No. 3 ·2005-10-00 ·Pages 235-47

Kiparisov S, Petrov A, Meskauskas A, Sergiev PV, Dontsova OA, Dinman JD

Abstract

5S rRNA extends from the central protuberance of the large ribosomal subunit, through the A-site finger, and down to the GTPase-associated center. Here, we present a structure-function analysis of seven 5S rRNA alleles which are sufficient for viability in the yeast Saccharomyces cerevisiae when expressed in the absence of wild-type 5S rRNAs, and extend this analysis using a large bank of mutant alleles that show semi-dominant phenotypes in the presence of wild-type 5S rRNA. This analysis supports the hypothesis that 5S rRNA serves to link together several different functional centers of the ribosome. Data are also presented which suggest that in eukaryotic genomes selection has favored the maintenance of multiple alleles of 5S rRNA, and that these may provide cells with a mechanism to post-transcriptionally regulate gene expression.

MeSH Terms
Alleles Base Pairing Base Sequence Frameshifting, Ribosomal/genetics Models, Molecular Molecular Sequence Data Phenotype Plasmids/genetics RNA, Ribosomal, 5S/chemistry,genetics Ribosomes/genetics Saccharomyces cerevisiae/genetics Sequence Alignment Sequence Analysis, DNA
Chemicals
RNA, Ribosomal, 5S
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kiparisov Sergey
Department of Chemistry, Moscow State University, Russia.
Petrov Alexey
Meskauskas Arturas
Sergiev Petr V
Dontsova Olga A
Dinman Jonathan D
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Article Info
Journal
Molecular genetics and genomics : MGG
Abbr.
Mol Genet Genomics
ISSN
1617-4615
DOI
10.1007/s00438-005-0020-9
Published
2005-10-00
Epub
2005-00-20
Pages
235-47
Language
English
Region
Germany
NLM ID
101093320
PMCID
PMC1276653
Subset
IM
Grants
NIGMS NIH HHS · R01 GM062143 · United States
FIC NIH HHS · R03 TW005787 · United States
NIGMS NIH HHS · GM62143 · United States
FIC NIH HHS · TW005787 · United States
External Links
PubMed source DOI: 10.1007/s00438-005-0020-9 PMC Full Text View journal details
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