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PMID: 17194937 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Ribosomal protein L3: influence on ribosome structure and function.

RNA biology ·Vol. 1 ·No. 1 ·2004-05-00 ·Pages 59-65

Petrov A, Meskauskas A, Dinman JD

Abstract

Early studies demonstrated roles for ribosomal protein L3 in peptidyltransferase center formation and the ability of cells to propagate viruses. More recent studies have linked these two processes via the effects of mutants and drugs on programmed -1 ribosomal frameshifting. Here, we show that mutant forms of L3 result in ribosomes having increased affinities for both aminoacyl- and peptidyl-tRNAs. These defects potentiate the effects of sparsomycin, which promotes increased aminoalcyl-tRNA binding at the P-site, while antagonizing the effects anisomycin, a drug that promotes decreased peptidyl-tRNA binding at the A-site. The changes in ribosome affinities for tRNAs also correlate with decreased peptidyltransferase activities of mutant ribosomes, and with decreased rates of cell growth and protein synthesis. In vivo dimethylsulfate (DMS) protection studies reveal that small changes in L3 primary sequence also have significant effects on rRNA structure as far away as 100 A, supporting an allosteric model of ribosome function.

MeSH Terms
Allosteric Site Anisomycin/pharmacology Base Sequence Gene Expression Regulation Haloarcula marismortui/metabolism Kinetics Molecular Sequence Data Mutation Nucleic Acid Conformation RNA Stability RNA, Ribosomal/chemistry Ribosomal Protein L3 Ribosomal Proteins/metabolism,physiology Ribosomes/chemistry Sparsomycin/pharmacology Sulfuric Acid Esters/pharmacology
Chemicals
RNA, Ribosomal Ribosomal Protein L3 Ribosomal Proteins Sulfuric Acid Esters Anisomycin Sparsomycin dimethyl sulfate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Petrov Alexey
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742, USA.
Meskauskas Arturas
Dinman Jonathan D
References (33)
33 references, click to expand
  1. Ribosomal proteins L15 and L16 are mere late assembly proteins of the large ribosomal subunit. Analysis of an Escherichia coli mutant lacking L15.
    J Biol Chem. 1990 Sep 25;265(27):16676-82 PMID: 2204629
  2. Transfer RNA binding to 80S ribosomes from yeast: evidence for three sites.
    Biochem Mol Biol Int. 1994 Aug;33(5):909-15 PMID: 7987260
  3. Ribosomes and translation.
    Annu Rev Biochem. 1997;66:679-716 PMID: 9242921
  4. An in vivo and in vitro structure-function analysis of the Saccharomyces cerevisiae U3A snoRNP: protein-RNA contacts and base-pair interaction with the pre-ribosomal RNA.
    J Mol Biol. 1997 Oct 31;273(3):552-71 PMID: 9356246
  5. A new nomenclature for the cytoplasmic ribosomal proteins of Saccharomyces cerevisiae.
    Nucleic Acids Res. 1997 Dec 15;25(24):4872-5 PMID: 9396790
  6. Ribosomal protein L3 mutants alter translational fidelity and promote rapid loss of the yeast killer virus.
    Mol Cell Biol. 1999 Jan;19(1):384-91 PMID: 9858562
  7. Direct crosslinking of the antitumor antibiotic sparsomycin, and its derivatives, to A2602 in the peptidyl transferase center of 23S-like rRNA within ribosome-tRNA complexes.
    Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):9003-8 PMID: 10430885
  8. Peptidyl transferase antibiotics perturb the relative positioning of the 3'-terminal adenosine of P/P'-site-bound tRNA and 23S rRNA in the ribosome.
    RNA. 1999 Aug;5(8):1003-13 PMID: 10445875
  9. The 3D arrangement of the 23 S and 5 S rRNA in the Escherichia coli 50 S ribosomal subunit based on a cryo-electron microscopic reconstruction at 7.5 A resolution.
    J Mol Biol. 2000 Apr 21;298(1):35-59 PMID: 10756104
  10. The complete atomic structure of the large ribosomal subunit at 2.4 A resolution.
    Science. 2000 Aug 11;289(5481):905-20 PMID: 10937989
  11. Transit of tRNA through the Escherichia coli ribosome. Cross-linking of the 3' end of tRNA to specific nucleotides of the 23 S ribosomal RNA at the A, P, and E sites.
    J Biol Chem. 2000 Dec 1;275(48):37887-94 PMID: 10961994
  12. Crystal structure of the ribosome at 5.5 A resolution.
    Science. 2001 May 4;292(5518):883-96 PMID: 11283358
  13. Saturation mutagenesis of 5S rRNA in Saccharomyces cerevisiae.
    Mol Cell Biol. 2001 Dec;21(24):8264-75 PMID: 11713264
  14. An "integrated model" of programmed ribosomal frameshifting.
    Trends Biochem Sci. 2002 Sep;27(9):448-54 PMID: 12217519
  15. The 9-A solution: how mRNA pseudoknots promote efficient programmed -1 ribosomal frameshifting.
    RNA. 2003 Feb;9(2):168-74 PMID: 12554858
  16. Delayed rRNA processing results in significant ribosome biogenesis and functional defects.
    Mol Cell Biol. 2003 Mar;23(5):1602-13 PMID: 12588980
  17. Decreased peptidyltransferase activity correlates with increased programmed -1 ribosomal frameshifting and viral maintenance defects in the yeast Saccharomyces cerevisiae.
    RNA. 2003 Aug;9(8):982-92 PMID: 12869709
  18. RNA, the first macromolecular catalyst: the ribosome is a ribozyme.
    Trends Biochem Sci. 2003 Aug;28(8):411-8 PMID: 12932729
  19. P-site tRNA is a crucial initiator of ribosomal frameshifting.
    RNA. 2004 Feb;10(2):221-30 PMID: 14730021
  20. Stabilization of N-acetylphenylalanyl transfer ribonucleic acid binding to ribosomes by sparsomycin.
    Biochemistry. 1969 Apr;8(4):1335-44 PMID: 4896459
  21. Studies on the formation of transfer ribonucleic acid-ribosome complexes. XXIV. Effects of antibiotics on binding of aminoacyl-oligonucleotides to ribosomes.
    J Biol Chem. 1973 Feb 25;248(4):1168-74 PMID: 4568810
  22. Trichodermin resistance--mutation affecting eukaryotic ribosomes.
    Nature. 1974 Apr 5;248(448):535-6 PMID: 4596184
  23. Chromosomal and nonchromosomal mutations affecting the "killer character" of Saccharomyces cerevisiae.
    Genetics. 1974 Mar;76(3):423-32 PMID: 4364866
  24. Simultaneous ribosomal resistance to trichodermin and anisomycin in Saccharomyces cerevisiae mutants.
    Biochim Biophys Acta. 1975 Apr 2;383(4):427-34 PMID: 1092352
  25. Mapping of trichodermin resistance in Saccharomyces cerevisiae: a genetic locus for a component of the 60S ribsomal subunit.
    Genetics. 1976 Aug;83(4):667-73 PMID: 786781
  26. Electron microscopic heteroduplex analysis of "killer" double-stranded RNA species from yeast.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4224-8 PMID: 360211
  27. Assembly map of the 50-S subunit from Escherichia coli ribosomes, covering the proteins present in the first reconstitution intermediate particle.
    Eur J Biochem. 1980 Jan;103(1):95-8 PMID: 6153613
  28. Ribosomal components from Escherichia coli 50 S subunits involved in the reconstitution of peptidyltransferase activity.
    J Biol Chem. 1981 Mar 10;256(5):2284-8 PMID: 7007380
  29. Cloning of yeast gene for trichodermin resistance and ribosomal protein L3.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):238-42 PMID: 7017711
  30. Ribosomal protein L3 is involved in replication or maintenance of the killer double-stranded RNA genome of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1982 Aug;79(15):4706-8 PMID: 6750608
  31. Nucleotide sequence of the tcml gene (ribosomal protein L3) of Saccharomyces cerevisiae.
    J Bacteriol. 1983 Jul;155(1):8-14 PMID: 6305925
  32. Minimal set of ribosomal components for reconstitution of the peptidyltransferase activity.
    EMBO J. 1982;1(5):609-13 PMID: 6765232
  33. Conformation of yeast 18S rRNA. Direct chemical probing of the 5' domain in ribosomal subunits and in deproteinized RNA by reverse transcriptase mapping of dimethyl sulfate-accessible.
    Nucleic Acids Res. 1985 Dec 9;13(23):8339-57 PMID: 2417197
Article Info
Journal
RNA biology
Abbr.
RNA Biol
ISSN
1555-8584
DOI
10.4161/rna.1.1.957
Published
2004-05-00
Epub
2004-00-01
Pages
59-65
Language
English
Region
United States
NLM ID
101235328
PMCID
PMC1989672
Subset
IM
Grants
NIGMS NIH HHS · R01 GM062143-04 · United States
NIGMS NIH HHS · R01 GM058859 · United States
NIGMS NIH HHS · R01 GM058859-05 · United States
NIGMS NIH HHS · R01 GM058859-06 · United States
NIGMS NIH HHS · GM62143 · United States
NIGMS NIH HHS · R01 GM058859-04 · United States
NIGMS NIH HHS · R01 GM062143-03 · United States
NIGMS NIH HHS · R01 GM58859 · United States
NIGMS NIH HHS · R01 GM062143 · United States
External Links
PubMed source DOI: 10.4161/rna.1.1.957 PMC Full Text View journal details
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